Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis

Morse, Daniel J.; Smith, Ann; Wilson, Melanie J.; Marsh, Lucy; White, Lewis; Posso, Raquel; Bradshaw, David J.; Wei, Xiaoqing; Lewis, Michael A. O.; Williams, David W.

Abstract

Denture-associated stomatitis (DS) affects over two-thirds of denture-wearers. DS presents as erythema of the palatal mucosa in areas where denture-surface associated polymicrobial biofilms containing the fungus Candida albicans exist. The contribution of the oral bacterial microbiota toward the infection is unknown. Therefore, this study characterised the bacterial microbiota of sites within the oral cavity to identify potential associations with occurrence of DS. Denture-wearing patients were recruited (denture stomatitis (DS) n = 8; non-denture stomatitis (NoDS) n = 11) and the oral bacterial microbiota of the tongue, palate and denture-fitting surface was characterised using next-generation sequencing. Operational taxonomic units (OTUs) were identified to bacterial genera and species, and presence/absence and relative abundances were examined. A significant (P = 0.007) decrease in the number of OTUs and thus, diversity of the microbiota was observed in tongue samples of DS patients (vs non-DS). The microbiota of denture-fitting surfaces and palatal mucosae were similar. Large differences in the abundance of bacterial genera and species were observed at each sample site, and unique presence/absence of bacteria was noted. Presence/absence and relative abundance of specific bacteria associated with DS warrants further in vitro and in vivo evaluation, particularly as our previous work has shown C. albicans virulence factor modulation by oral bacteria.

Citation

Morse, D. J., Smith, A., Wilson, M. J., Marsh, L., White, L., Posso, R., …Williams, D. W. (2019). Molecular community profiling of the bacterial microbiota associated with denture-related stomatitis. Scientific Reports, 9(1), Article 10228. https://doi.org/10.1038/s41598-019-46494-0

• Journal Article Type: Article
• Acceptance Date: Jun 27, 2019
• Online Publication Date: Jul 15, 2019
• Publication Date: Dec 1, 2019
• Deposit Date: Oct 2, 2019
• Publicly Available Date: Oct 4, 2019
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Research (part of Springer Nature)
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 1
• Article Number: 10228
• DOI: https://doi.org/10.1038/s41598-019-46494-0
• Keywords: Multidisciplinary
• Public URL: https://uwe-repository.worktribe.com/output/3429011
• Publisher URL: https://www.nature.com/articles/s41598-019-46494-0
• Additional Information: Received: 22 January 2019; Accepted: 27 June 2019; First Online: 15 July 2019; : This work was financially supported by an Engineering and Physical Sciences Research Council and GlaxoSmithKline Consumer Healthcare case-award PhD studentship, grant number EP/L505390/1. The funders had no specific role in the conceptualisation, design, data collection or analysis, nor the decision to publish the work. Dr David Bradshaw is employed by GlaxoSmithKline Consumer Healthcare. No other personal or company-associated financial interests to declare. No non-financial competing interests to declare.