M. K. Cheung
Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns
Cheung, M. K.; Burns, C.; Gregory, K. E.; Kirby, M.; Riquelme, M.; Elliott, T. J.; Challen, M. P.; Bailey, A.; Foster, G. D.
Authors
C. Burns
K. E. Gregory
M. Kirby
M. Riquelme
T. J. Elliott
M. P. Challen
A. Bailey
G. D. Foster
Abstract
We have developed a "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the C. cinereus trp1 promoter and A. bisporus trp2 and gpdII promoters proving successful in driving expression in C. cinereus, with the gpdII promoter also functioning in A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron. This is the first reported expression of GFP in either C. cinereus or A. bisporus. © 2004 Elsevier Inc. All rights reserved.
Citation
Cheung, M. K., Burns, C., Gregory, K. E., Kirby, M., Riquelme, M., Elliott, T. J., …Foster, G. D. (2005). Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns. Fungal Genetics and Biology, 42(3), 191-199. https://doi.org/10.1016/j.fgb.2004.11.005
Journal Article Type | Article |
---|---|
Publication Date | Jan 1, 2005 |
Journal | Fungal Genetics and Biology |
Print ISSN | 1087-1845 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 42 |
Issue | 3 |
Pages | 191-199 |
DOI | https://doi.org/10.1016/j.fgb.2004.11.005 |
Public URL | https://uwe-repository.worktribe.com/output/1054209 |
Publisher URL | http://dx.doi.org/10.1016/j.fgb.2004.11.005 |
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