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Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns

Cheung, M. K.; Burns, C.; Gregory, K. E.; Kirby, M.; Riquelme, M.; Elliott, T. J.; Challen, M. P.; Bailey, A.; Foster, G. D.

Authors

M. K. Cheung

C. Burns

K. E. Gregory

M. Kirby

M. Riquelme

T. J. Elliott

M. P. Challen

A. Bailey

G. D. Foster



Abstract

We have developed a "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the C. cinereus trp1 promoter and A. bisporus trp2 and gpdII promoters proving successful in driving expression in C. cinereus, with the gpdII promoter also functioning in A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron. This is the first reported expression of GFP in either C. cinereus or A. bisporus. © 2004 Elsevier Inc. All rights reserved.

Journal Article Type Article
Publication Date Jan 1, 2005
Journal Fungal Genetics and Biology
Print ISSN 1087-1845
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 42
Issue 3
Pages 191-199
DOI https://doi.org/10.1016/j.fgb.2004.11.005
Public URL https://uwe-repository.worktribe.com/output/1054209
Publisher URL http://dx.doi.org/10.1016/j.fgb.2004.11.005


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