Metal oxide decorated carbon nanocomposite electrodes for propofol monitoring

Ferrier, David; Kiely, Janice; Luxton, Richard

Abstract

Despite the growing evidence of the benefits of total-intravenous anaesthesia using propofol compared to conventional volatile-based anaesthesia, both in terms of environmental impact and patient outcomes, the majority of administered general anaesthetics use volatile agents. A significant reason for this is the lack of suitable methods for continuous, real-time propofol monitoring. Here we present a cytochrome P450 2B6/carbon nanotube/graphene oxide/metal oxide nanocomposite sensor for propofol monitoring. The enzyme prevents electrode fouling by converting the propofol into a quinone/quinol redox pair and the nanocomposite enables rapid and sensitive detection. The nanocomposite was synthesised via a simple ‘green synthesis’-based approach using an extract of common bay laurel. It was found that composites containing iron oxide nanoparticles resulted in the best performance, with a limit of detection of 7.0 ± 0.7 ng/ml and a sensitivity of 29.9 ± 6.4 nA/μg/ml/mm2. The sensor demonstrated good specificity with respect to several common perioperative drugs, propofol detection was demonstrated in a ‘serum-like’ solution and produced a linear response across the therapeutic range of propofol (1–10 μg/ml).

• Journal Article Type: Article
• Acceptance Date: Nov 22, 2022
• Online Publication Date: Nov 29, 2022
• Publication Date: Dec 1, 2022
• Deposit Date: Dec 5, 2022
• Publicly Available Date: Dec 5, 2022
• Journal: Biosensors and Bioelectronics: X
• Print ISSN: 0956-5663
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Article Number: 100286
• DOI: https://doi.org/10.1016/j.biosx.2022.100286
• Keywords: Propofol biosensor; 2,6-diisopropylphenol sensor; metal oxide nanoparticle; nanocomposite; green synthesis; electrochemical detection
• Public URL: https://uwe-repository.worktribe.com/output/10222268
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S2590137022001790?via%3Dihub