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Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Langford, Geraint J.; Raeburn, Jaclyn; Ferrier, David C.; Hands, Philip J. W.; Shaver, Michael P.

Authors

Geraint J. Langford

Jaclyn Raeburn

David Ferrier David.Ferrier@uwe.ac.uk
Research Fellow- Biosensing/ Health Technology

Philip J. W. Hands

Michael P. Shaver



Abstract

© 2018 American Chemical Society. Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive cross-links for microRNA sequence detection. The MO cross-links can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO cross-links offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

Journal Article Type Article
Acceptance Date Dec 28, 2018
Online Publication Date Dec 28, 2018
Publication Date Jan 25, 2019
Deposit Date Jan 25, 2019
Journal ACS Sensors
Print ISSN 2379-3694
Electronic ISSN 2379-3694
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
Pages 185-191
DOI https://doi.org/10.1021/acssensors.8b01208
Keywords morpholino oligonucleotide, oligonucleotide sensor, biosensor, hydrogel, microRNA
Public URL https://uwe-repository.worktribe.com/output/853100
Publisher URL http://doi.org/10.1021/acssensors.8b01208
Additional Information Additional Information : This is the author's accepted manuscript. The final published version is available here: http://doi.org/10.1021/acssensors.8b01208.
Contract Date Jan 25, 2019

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