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Nanoparticle-based 3D membrane for impedimetric biosensor applications

Cao, Lu; Kiely, Janice; Piano, Martina; Luxton, Richard

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Lu Cao

Janice Kiely
Professor in Bio-electronics/Res In CoDi

Martina Piano
Research Fellow Biosensor reproducibility testing

Richard Luxton
Research Centre Director-IBST/Professor


This paper reports on a comparison between nano-ZnO/CuO and nano-ZnO nitrocellulose membrane biosensors, both of which were fabricated using a simple and inexpensive sonication technique. To produce the nano-ZnO/CuO membranes, the technique involved sonication of 1% (w/v) ZnO and 1% (w/v) CuO nano-crystal colloidal suspensions, with a volume ratio of 1:2. The membranes were analysed by scanning electron microscopy and energy-dispersive spectroscopy, which showed the gradated distribution of nanoparticles in the membrane. Impedance spectroscopy demonstrated that the sonication resulted in a greater than two-fold enhancement of the output signal. Changes in impedance phase values, at a frequency of 100 Hz, were used to establish dose dependent responses for C-reactive protein (CRP). Limits of detection of 27 pg/mL for the 1% (w/v) nano-ZnO and 16 pg/mL for the 1% (w/v) nano-ZnO/CuO nitrocellulose membrane biosensors were demonstrated.


Cao, L., Kiely, J., Piano, M., & Luxton, R. (2020). Nanoparticle-based 3D membrane for impedimetric biosensor applications. Bioelectrochemistry, 136,

Journal Article Type Article
Acceptance Date Jun 28, 2020
Online Publication Date Jul 3, 2020
Publication Date Dec 1, 2020
Deposit Date Sep 24, 2020
Publicly Available Date Jul 4, 2021
Journal Bioelectrochemistry
Print ISSN 1567-5394
Electronic ISSN 1878-562X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 136
Article Number 107593
Keywords Physical and Theoretical Chemistry; Biophysics; Electrochemistry; General Medicine; ZnO/CuO nitrocellulose membrane; Impedance spectroscopy; Phase change; CRP detection
Public URL
Additional Information This article is maintained by: Elsevier; Article Title: Nanoparticle-based 3D membrane for impedimetric biosensor applications; Journal Title: Bioelectrochemistry; CrossRef DOI link to publisher maintained version:; Content Type: article; Copyright: © 2020 Elsevier B.V. All rights reserved.


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