Nanoparticle-based 3D membrane for impedimetric biosensor applications

Cao, Lu; Kiely, Janice; Piano, Martina; Luxton, Richard

Abstract

This paper reports on a comparison between nano-ZnO/CuO and nano-ZnO nitrocellulose membrane biosensors, both of which were fabricated using a simple and inexpensive sonication technique. To produce the nano-ZnO/CuO membranes, the technique involved sonication of 1% (w/v) ZnO and 1% (w/v) CuO nano-crystal colloidal suspensions, with a volume ratio of 1:2. The membranes were analysed by scanning electron microscopy and energy-dispersive spectroscopy, which showed the gradated distribution of nanoparticles in the membrane. Impedance spectroscopy demonstrated that the sonication resulted in a greater than two-fold enhancement of the output signal. Changes in impedance phase values, at a frequency of 100 Hz, were used to establish dose dependent responses for C-reactive protein (CRP). Limits of detection of 27 pg/mL for the 1% (w/v) nano-ZnO and 16 pg/mL for the 1% (w/v) nano-ZnO/CuO nitrocellulose membrane biosensors were demonstrated.

• Journal Article Type: Article
• Acceptance Date: Jun 28, 2020
• Online Publication Date: Jul 3, 2020
• Publication Date: Dec 1, 2020
• Deposit Date: Sep 24, 2020
• Publicly Available Date: Jul 4, 2021
• Journal: Bioelectrochemistry
• Print ISSN: 1567-5394
• Electronic ISSN: 1878-562X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 136
• Article Number: 107593
• DOI: https://doi.org/10.1016/j.bioelechem.2020.107593
• Keywords: Physical and Theoretical Chemistry; Biophysics; Electrochemistry; General Medicine; ZnO/CuO nitrocellulose membrane; Impedance spectroscopy; Phase change; CRP detection
• Public URL: https://uwe-repository.worktribe.com/output/6476654
• Additional Information: This article is maintained by: Elsevier; Article Title: Nanoparticle-based 3D membrane for impedimetric biosensor applications; Journal Title: Bioelectrochemistry; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.bioelechem.2020.107593; Content Type: article; Copyright: © 2020 Elsevier B.V. All rights reserved.