Selective and rapid biosensor integrated into a commercial hand-held instrument for the measurement of ammonium ion in sewage effluent

Hart, John P.; Serban, S.; Jones, L. J.; Biddle, N.; Drago, Guido A.; Pittson, R.

Abstract

A disposable biosensor for ammonium ions in sewage effluent was developed and based on a screen-printed carbon electrode coated with the enzyme glutamate dehydrogenase, 2-oxoglutarate, and NADH. This rapid and selective amperometric biosensor is capable of detecting NH4+ in sewage effluent at concentrations in the range 1-10 ppm. The biosensor was developed as a disposable, reagentless device incorporated into a fully automated, hand-held instrument that can be used away from the laboratory. This low cost, commercially available system permits the measurement of NH4+ in effluent samples in only 5 min and 20 s without any pretreatment. The results obtained correlated well with the standard laboratory method, used by the Environment Agency. Copyright © Taylor & Francis Group, LLC.

• Presentation Conference Type: Conference Paper (published)
• Publication Date: Jun 20, 2006
• Journal: Analytical Letters
• Print ISSN: 0003-2719
• Electronic ISSN: 1532-236X
• Publisher: Taylor & Francis
• Peer Reviewed: Not Peer Reviewed
• Volume: 39
• Issue: 8
• Pages: 1657-1667
• DOI: https://doi.org/10.1080/00032710600713545
• Keywords: screen‐printed electrode, amperometric biosensor, ; ammonium ion, sewage effluent
• Public URL: https://uwe-repository.worktribe.com/output/1044063