Outputs (46)

The value of twinned pollinator-pollen metabarcoding: Bumblebee pollination service is weakly partitioned within a UK grassland community (2023)
Journal Article
Ronca, S., Ford, C. S., Allanguillame, J., Szabo, C., Kipling, R., & Wilkinson, M. J. (2023). The value of twinned pollinator-pollen metabarcoding: Bumblebee pollination service is weakly partitioned within a UK grassland community. Scientific Reports, 13(1), Article 18016. https://doi.org/10.1038/s41598-023-44822-z

Predicting ecological impact of declining bumblebee (Bombus) populations requires better understanding of interactions between pollinator partitioning of floral resources and plant partitioning of pollinator resources. Here, we combine Cytochrome Oxi... Read More about The value of twinned pollinator-pollen metabarcoding: Bumblebee pollination service is weakly partitioned within a UK grassland community.

Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK (2022)
Journal Article
Kile, H., Arnold, D., Allainguillaume, J., Denman, S., & Brady, C. (2022). Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK. International Journal of Systematic and Evolutionary Microbiology, 72(10), https://doi.org/10.1099/ijsem.0.005515

Several strains of a previously undescribed bacterial species were isolated from mature Tilia hybrid trees suffering from bleed-ing cankers at various geographic locations in the UK. The strains were Gram-negative, facultatively anaerobic, and partia... Read More about Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK.

Brassica and Sinapis seeds in medieval archaeological sites: An example of multiproxy analysis for their identification and ethnobotanical interpretation (2022)
Journal Article
Bosi, G., De Felice, S., Wilkinson, M. J., Allainguillaume, J., Arru, L., Nascimbene, J., & Buldrini, F. (2022). Brassica and Sinapis seeds in medieval archaeological sites: An example of multiproxy analysis for their identification and ethnobotanical interpretation. Plants, 11(16), 2100. https://doi.org/10.3390/plants11162100

The genus Brassica includes some of the most important vegetable and oil crops worldwide. Many Brassica seeds (which can show diagnostic characters useful for species identification) were recovered from two archaeological sites in northern Italy, dat... Read More about Brassica and Sinapis seeds in medieval archaeological sites: An example of multiproxy analysis for their identification and ethnobotanical interpretation.

Broken chocolate: Biomarkers as a method for delivering cocoa supply chain visibility (2021)
Journal Article
Lafargue, P., Rogerson, M., Parry, G. C., & Allainguillaume, J. (2022). Broken chocolate: Biomarkers as a method for delivering cocoa supply chain visibility. Supply Chain Management, 27(6), 728-741. https://doi.org/10.1108/SCM-11-2020-0583

Purpose: This paper examines the potential of “biomarkers” to provide immutable identification for food products (chocolate), providing traceability and visibility in the supply chain from retail product back to farm. Design/methodology/approach: Thi... Read More about Broken chocolate: Biomarkers as a method for delivering cocoa supply chain visibility.

Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao) (2021)
Journal Article
Muller, E., Ullah, I., Dunwell, J. M., Daymond, A. J., Richardson, M., Allainguillaume, J., & Wetten, A. (2021). Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao). Scientific Reports, 11(1), Article 8270. https://doi.org/10.1038/s41598-021-87690-1

Theobroma cacao is one of the most economically important tropical trees, being the source of chocolate. As part of an ongoing study to understand the diversity of the badnavirus complex, responsible for the cacao swollen shoot virus disease in West... Read More about Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao).

Sensor (2020)
Patent
Allainguillaume, J., Barnett, J., Luxton, R., & Kiely, J. Sensor. WO 2020/089607 A1

The present invention relates to detecting viral infections in plants, especially Cacao swollen-shoot virus (CSSV) infections. Recombinant viral coat protein antigens and binding reagents that specifically bind to thereto are provided together with a... Read More about Sensor.

Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas (2020)
Journal Article
Bueno-Gonzalez, V., Brady, C., Denman, S., Allainguillaume, J., & Arnold, D. (2020). Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas. International Journal of Systematic and Evolutionary Microbiology, 70(4), 2426-2434. https://doi.org/10.1099/ijsem.0.004055

As the current episode of Acute Oak Decline (AOD) continues to affect native British oak in the United Kingdom, ongoing isola-tions from symptomatic and healthy oak have yielded a large Pseudomonas species population. These strains could be divided i... Read More about Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas.

Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK (2019)
Journal Article
Bueno-Gonzalez, V., Brady, C., Denman, S., Plummer, S., Allainguillaume, J., & Arnold, D. (2019). Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK. International Journal of Systematic and Evolutionary Microbiology, 69(11), 3368-3376. https://doi.org/10.1099/ijsem.0.003615

Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase pos... Read More about Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK.

CSSV biosensor (WO2020089607-PAMPH-20200507-8395) (2019)
Patent
Allainguillaume, J., Barnett, J. M., Kiely, J., & Luxton, R. (in press). CSSV biosensor (WO2020089607-PAMPH-20200507-8395). WO 2020/089607 A1

The present invention relates to detecting viral infections in plants, especially Cacao swollen-shoot virus (CSSV) infections. Recombinant viral coat protein antigens and binding reagents that specifically bind to thereto are provided together with a... Read More about CSSV biosensor (WO2020089607-PAMPH-20200507-8395).

Lemurs in Cacao: Presence and Abundance within the Shade Plantations of Northern Madagascar (2019)
Journal Article
Webber, A. D., Solofondranohatra, J. S., Razafindramoana, S., Fernandez, D., Parker, C. A., Steer, M., …Allainguillaume, J. (in press). Lemurs in Cacao: Presence and Abundance within the Shade Plantations of Northern Madagascar. Folia Primatologica, 91(2), 96-107. https://doi.org/10.1159/000501987

© 2019 S. Karger AG, Basel. The recognition that much biodiversity exists outside protected areas is driving research to understand how animals survive in anthropogenic landscapes. In Madagascar, cacao (Theobroma cacao) is grown under a mix of native... Read More about Lemurs in Cacao: Presence and Abundance within the Shade Plantations of Northern Madagascar.

Investigating ROS, RNS, and H2S-sensitive signaling proteins (2019)
Book Chapter
Williams, E., Whiteman, M., Wood, M. E., Wilson, I. D., Ladomery, M. R., Allainguillaume, J., …Hancock, J. T. (2019). Investigating ROS, RNS, and H2S-sensitive signaling proteins. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction (27-42). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_3

© Springer Science+Business Media, LLC, part of Springer Nature 2019. The modification of proteins is a key way to alter their activity and function. Often thiols, cysteine residues, on proteins are attractive targets for such modification. Assuming... Read More about Investigating ROS, RNS, and H2S-sensitive signaling proteins.

The use of chloroplast markers for the traceability of certified sustainably produced cacao (Theobroma cacao) in the chocolate industry (2018)
Conference Proceeding
Allainguillaume, J., & wetten, A. (2018). The use of chloroplast markers for the traceability of certified sustainably produced cacao (Theobroma cacao) in the chocolate industry. In International Cocoa Symposium 2017 Proceedings

Recently the chocolate industry has changed to a higher demand for sustainably certified cacao (Rainforest Alliance, UTZ, and FairTrade) and closer attention is being paid to how this sustainably produced cocoa can be traced. Companies like Mars, Her... Read More about The use of chloroplast markers for the traceability of certified sustainably produced cacao (Theobroma cacao) in the chocolate industry.

Electropenetrography application and molecular-based virus detection in mealybug (Hemiptera: Pseudococcidae) vectors of Cacao swollen shoot virus on Theobroma cacao L. (2018)
Journal Article
Obok, E., Wetten, A., & Allainguillaume, J. (2018). Electropenetrography application and molecular-based virus detection in mealybug (Hemiptera: Pseudococcidae) vectors of Cacao swollen shoot virus on Theobroma cacao L. Annals of Agricultural Sciences, 63(1), 55-65. https://doi.org/10.1016/j.aoas.2018.04.004

© 2018 Cacao swollen shoot virus (CSSV) is a peril exclusive to the West African cacao-growing countries; causing the Cacao swollen shoot virus disease. This study was set out (1) to analyse the feeding behaviour of two West African and one non-West... Read More about Electropenetrography application and molecular-based virus detection in mealybug (Hemiptera: Pseudococcidae) vectors of Cacao swollen shoot virus on Theobroma cacao L..

Molecular evidence of Cacao swollen shoot virus acquisition and retention by Planococcus citri (Risso) and Pseudococcus longispinus (Targioni-Tozzetti) and Pseudococcus viburni (Signoret) Mealybugs (Hemiptera: Pseudococcidae) (2018)
Journal Article
Allainguillaume, J., Wetten, A., & Obok, E. (2018). Molecular evidence of Cacao swollen shoot virus acquisition and retention by Planococcus citri (Risso) and Pseudococcus longispinus (Targioni-Tozzetti) and Pseudococcus viburni (Signoret) Mealybugs (Hemiptera: Pseudococcidae). https://doi.org/10.29322/IJSRP.8.3.2018.p7507

Cacao swollen shoot virus (CSSV) remains a significant viral pathogen endemic in West African cacao-growing countries. Eradication of affected trees (complete removal) and resistance breeding in existing cacao (Theobroma cacao L.) germplasm have not... Read More about Molecular evidence of Cacao swollen shoot virus acquisition and retention by Planococcus citri (Risso) and Pseudococcus longispinus (Targioni-Tozzetti) and Pseudococcus viburni (Signoret) Mealybugs (Hemiptera: Pseudococcidae).

Next generation sequencing elucidates cacao badnavirus diversity and reveals the existence of more than ten viral species (2017)
Journal Article
Muller, E., Ravel, S., Agret, C., Abrokwah, F., Dzahini-Obiatey, H., Galyuon, I., …Wetten, A. (2018). Next generation sequencing elucidates cacao badnavirus diversity and reveals the existence of more than ten viral species. Virus Research, 244, 235-251. https://doi.org/10.1016/j.virusres.2017.11.019

© 2017 Elsevier B.V. Cacao swollen shoot virus is a member of the family Caulimoviridae, genus Badnavirus and is naturally transmitted to Theobroma cacao (L.) by several mealybug species. CSSV populations in West African countries are highly variable... Read More about Next generation sequencing elucidates cacao badnavirus diversity and reveals the existence of more than ten viral species.

Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans (2017)
Journal Article
Floyd, K. G., Hopewell, H., Burnell, D., Hancock, J. T., Allainguillaume, J., Ladomery, M., & Wilson, I. D. (2017). Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans. Ecotoxicology, 26(7), 981-990. https://doi.org/10.1007/s10646-017-1826-z

© 2017, The Author(s). This study investigated the neurological effects of residual ground-water levels of thiaclopridon the non-target organism Caenorhabditis elegans. Nematodes treated with thiacloprid showed a dose-dependent and significantly incr... Read More about Residual ground-water levels of the neonicotinoid thiacloprid perturb chemosensing of Caenorhabditis elegans.

GM risk assessment: Pollen carriage from Brassica napus to B. rapa varies widely between pollinators (2017)
Journal Article
Wilkinson, M. J., Ford, C. S., Ronca, S., Allainguillaume, J., & Warren, J. (2017). GM risk assessment: Pollen carriage from Brassica napus to B. rapa varies widely between pollinators. Basic and Applied Ecology, 19, 36-44. https://doi.org/10.1016/j.baae.2017.01.007

© 2017 Gesellschaft für Ökologie Characterizing insect pollen carriage between closely related plant species is especially challenging where source species possess morphologically identical pollen and share many pollinators in common. Here, we use an... Read More about GM risk assessment: Pollen carriage from Brassica napus to B. rapa varies widely between pollinators.

Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis (2016)
Journal Article
Brady, C., Allainguillaume, J., Denman, S., & Arnold, D. (2016). Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis. Letters in Applied Microbiology, 63(2), 89-95. https://doi.org/10.1111/lam.12593

© 2016 The Society for Applied Microbiology Two Gram-negative Enterobacteriaceae, Gibbsiella quercinecans and Brenneria goodwinii, are frequently isolated from oak suffering from Acute Oak Decline. These two species are difficult to identify based on... Read More about Rapid identification of bacteria associated with Acute Oak Decline by high-resolution melt analysis.

A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells (2015)
Journal Article
May, J. E., Allainguillaume, J., Page, K., Stratford-Smith, C., Aruomaren, A., Alloush, H., …Anderson, E. (2015). A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells. Mutagenesis, 30(6), 856. https://doi.org/10.1093/mutage/gev074

Decitabine (DAC) and azacytidine (AZA) are cytidine ana- logues and hypomethylating agents used in the manage- ment of patients with myelodysplastic syndrome (MDS), where the main goal is to prevent transformation to acute myeloid leukaemia (AML), wh... Read More about A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells.

Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences (2015)
Journal Article
Hawkins, J., De Vere, N., Griffith, A., Ford, C. R., Allainguillaume, J., Hegarty, M. J., …Adams-Groom, B. (2015). Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences. PLoS ONE, 10(8), Article e0134735. https://doi.org/10.1371/journal.pone.0134735

Copyright © 2015 Hawkins et al. Identifying the floral composition of honey provides a method for investigating the plants that honey bees visit. We compared melissopalynology, where pollen grains retrieved from honey are identified morphologically,... Read More about Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences.

Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range (2015)
Journal Article
López-Alvarez, D., Manzaneda, A. J., Rey, P. J., Giraldo, P., Benavente, E., Allainguillaume, J., …Catalán, P. (2015). Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range. American Journal of Botany, 102(7), 1073-1088. https://doi.org/10.3732/ajb.1500128

© 2015 Botanical Society of America. PREMISE OF THE STUDY: We conducted environmental niche modeling (ENM) of the Brachypodium distachyon s.l. complex, a model group of two diploid annual grasses (B. distachyon, B. stacei) and their derived allotetra... Read More about Environmental niche variation and evolutionary diversification of the Brachypodium distachyon grass complex species in their native circum-mediterranean range.

High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread (2015)
Journal Article
Wetten, A., Campbell, C., & Allainguillaume, J. (2016). High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread. Pest Management Science, 72(3), 527-533. https://doi.org/10.1002/ps.4017

© 2015 Society of Chemical Industry. BACKGROUND: Mealybugs (Hemiptera: Coccoidea: Pseudococcidae) are key vectors of badnaviruses, including Cacao swollen shoot virus (CSSV), the most damaging virus affecting cacao (Theobroma cacao L.). The effective... Read More about High-resolution melt and morphological analyses of mealybugs (Hemiptera: Pseudococcidae) from cacao: Tools for the control of Cacao swollen shoot virus spread.

Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar (2014)
Journal Article
Ford, C. S., Allainguillaume, J., Fu, T. R., Mitchley, J., & Wilkinson, M. J. (2015). Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar. New Phytologist, 205(3), 1342-1349. https://doi.org/10.1111/nph.13131

© 2014 New Phytologist Trust. Paternal biocontainment methods (PBMs) act by preventing pollen-mediated transgene flow. They are compromised by transgene escape via the crop-maternal line. We therefore assess the efficacy of PBMs for transgenic rapese... Read More about Assessing the value of imperfect biocontainment nationally: Rapeseed in the United Kingdom as an exemplar.

Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination (2013)
Journal Article
Ameyaw, G. A., Wetten, A., Dzahini-Obiatey, H., Domfeh, O., & Allainguillaume, J. (2013). Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination. Plant Pathology, 62(2), 421-427. https://doi.org/10.1111/j.1365-3059.2012.02640.x

DNA- and RNA-based polymerase chain reaction (PCR) systems were used with Cacao swollen shoot virus (CSSV) primers designed from conserved regions of the six published genomic sequences of CSSV to investigate whether the virus is transmissible from i... Read More about Investigation on Cacao swollen shoot virus (CSSV) pollen transmission through cross-pollination.

Balancing selection and the maintenance of MHC supertype variation in a selfing vertebrate (2013)
Journal Article
Allainguillaume, J., Consuegra, S., Ellison, A., Allainguillaume, J., Pachebat, J., Peat, K. M., & Wright, P. (2013). Balancing selection and the maintenance of MHC supertype variation in a selfing vertebrate. Proceedings of the Royal Society B: Biological Sciences, 280(1754), 1-2. https://doi.org/10.1098/rspb.2012.2854

Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces (2012)
Patent
Allainguillaume, J., & Wilkinson, M. J. (2012). Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces. WO2012131300-A2 ; WO2012131300-A3

Abstract: NOVELTY - Method for analyzing sward, involves analyzing a sample of sward for the presence of one or more genetic markers, where the one or more genetic markers indicates the presence, identity and/or relative abundance of one or more spec... Read More about Analyzing sward and plant species make-up of sports and amenity turf, involves analyzing presence of genetic markers e.g. ribulose-bisphosphate carboxylase gene in sward sample obtained from sports or amenity turf surfaces.

DNA barcoding the native flowering plants and conifers of wales (2012)
Journal Article
Rich, T. C., Steinke, D., de Vere, N., Ford, C. R., Trinder, S. A., Long, C., …Wilkinson, M. J. (2012). DNA barcoding the native flowering plants and conifers of wales. PLoS ONE, 7(6), e37945. https://doi.org/10.1371/journal.pone.0037945

We present the first national DNA barcode resource that covers the native flowering plants and conifers for the nation of Wales (1143 species). Using the plant DNA barcode markers rbcL and matK, we have assembled 97.7% coverage for rbcL, 90.2% for ma... Read More about DNA barcoding the native flowering plants and conifers of wales.

Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate (2012)
Journal Article
Allainguillaume, J., Ellison, A., Allainguillaume, J., Girdwood, S., Pachebat, J., Peat, K. M., …Consuegra, S. (2012). Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate. Proceedings of the Royal Society B: Biological Sciences, 279(1749), 5004-5013. https://doi.org/10.1098/rspb.2012.1929

Major histocompatibility complex (MHC) genes encode proteins that present pathogen-derived antigens to T-cells, initiating the adaptive immune response in vertebrates. Although populations with low MHC diversity tend to be more susceptible to pathoge... Read More about Maintaining functional major histocompatibility complex diversity under inbreeding: The case of a selfing vertebrate.

Exploiting the brachypodium tool box in cereal and grass research (2011)
Journal Article
Mur, L. A., Allainguillaume, J., Catalán, P., Hasterok, R., Jenkins, G., Lesniewska, K., …Vogel, J. (2011). Exploiting the brachypodium tool box in cereal and grass research. New Phytologist, 191(2), 334-347. https://doi.org/10.1111/j.1469-8137.2011.03748.x

It is now a decade since Brachypodium distachyon (Brachypodium) was suggested as a model species for temperate grasses and cereals. Since then transformation protocols, large expressed sequence tag (EST) databases, tools for forward and reverse genet... Read More about Exploiting the brachypodium tool box in cereal and grass research.

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010 (2011)
Journal Article
Smulders, M. J., Reusch, T. B., Pinheiro, J. B., Nguyen, T. T., Messmer, A. M., Goodisman, M. A., …Zucchi, M. I. (2011). Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010. Molecular Ecology Resources, 11(1), 219-222. https://doi.org/10.1111/j.1755-0998.2010.02944.x

This article documents the addition of 229 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acacia auriculiformis-×-Acacia mangium hybrid, Alabama argillacea, Anoplopoma fimbria, A... Read More about Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2010 - 30 September 2010.

Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops (2009)
Journal Article
Elliott, L. M., Mason, D. C., Allainguillaume, J., & Wilkinson, M. J. (2009). Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops. Journal of Applied Remote Sensing, 3(1), 033562. https://doi.org/10.1117/1.3269615

High resolution descriptions of plant distribution have utility for many ecological applications but are especially useful for predictive modeling of gene flow from transgenic crops. Difficulty lies in the extrapolation errors that occur when limited... Read More about Use of airborne remote sensing to detect riverside Brassica rapa to aid in risk assessment of transgenic crops.

Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment (2009)
Journal Article
Allainguillaume, J., Harwood, T., Ford, C. S., Cuccato, G., Norris, C., Allender, C. J., …Wilkinson, M. J. (2009). Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment. New Phytologist, 183(4), 1201-1211. https://doi.org/10.1111/j.1469-8137.2009.02877.x

Biocontainment methods for genetically modified crops closest to commercial reality (chloroplast transformation, male sterility) would be compromised (in absolute terms) by seed-mediated gene flow leading to chloroplast capture. Even in these circums... Read More about Rapeseed cytoplasm gives advantage in wild relatives and complicates genetically modified crop biocontainment.

Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment (2009)
Journal Article
Haider, N., Allainguillaume, J., & Wilkinson, M. J. (2009). Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment. Current Genetics, 55(2), 139-150. https://doi.org/10.1007/s00294-009-0230-5

Environmental concerns over the cultivation of Genetically Modified (GM) crops largely centre on the ecological consequences following gene flow to wild relatives. One attractive solution is to deploy biocontainment measures that prevent hybridizatio... Read More about Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: Implications for the use of chloroplast transformation for biocontainment.

Transmission of cocoa swollen shoot virus by seeds (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). Transmission of cocoa swollen shoot virus by seeds. Journal of Virological Methods, 150(1-2), 45-49. https://doi.org/10.1016/j.jviromet.2008.03.009

A study was undertaken to determine whether cocoa swollen shoot virus is transmitted by seeds, to improve the robustness of quarantine procedures for international exchange and long term conservation of cocoa germplasm. PCR/capillary electrophoresis,... Read More about Transmission of cocoa swollen shoot virus by seeds.

The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees (2008)
Journal Article
Quainoo, A. K., Wetten, A. C., & Allainguillaume, J. (2008). The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149(1), 91-96. https://doi.org/10.1016/j.jviromet.2008.01.007

Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSS... Read More about The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.

Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives (2007)
Journal Article
Allender, C. J., Allainguillaume, J., Lynn, J., & King, G. J. (2007). Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives. TAG Theoretical and Applied Genetics, 114(4), 609-618. https://doi.org/10.1007/s00122-006-0461-5

Diversity in the chloroplast genome of 171 accessions representing the Brassica 'C' (n = 9) genome, including domesticated and wild B. oleracea and nine inter-fertile related wild species, was investigated using six chloroplast SSR (microsatellite) m... Read More about Simple sequence repeats reveal uneven distribution of genetic diversity in chloroplast genomes of Brassica oleracea L. and (n = 9) wild relatives.

Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea (2006)
Journal Article
Wilkinson, M. J., Allender, C. J., Ford, C. S., Allainguillaume, J., Grilli-Chantler, P., & Cuccato, G. (2006). Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea. Proceedings of the Royal Society B: Biological Sciences, 273(1605), 3111-3115. https://doi.org/10.1098/rspb.2006.3686

Research on the environmental risks of gene flow from genetically modified (GM) crops to wild relatives has traditionally emphasized recipients yielding most hybrids. For GM rapeseed (Brassica napus), interest has centred on the 'frequently hybridizi... Read More about Spontaneous gene flow from rapeseed (Brassica napus) to wild Brassica oleracea.

Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats (2006)
Journal Article
Allainguillaume, J., Alexander, M., Bullock, J. M., Saunders, M., Allender, C. J., King, G., …Wilkinson, M. J. (2006). Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats. Molecular Ecology, 15(4), 1175-1184. https://doi.org/10.1111/j.1365-294X.2006.02856.x

Fitness of hybrids between genetically modified (GM) crops and wild relatives influences the likelihood of ecological harm. We measured fitness components in spontaneous (non-GM) rapeseed x Brassica rapa hybrids in natural populations. The F1 hybrids... Read More about Fitness of hybrids between rapeseed (Brassica napus) and wild Brassica rapa in natural habitats.

Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA (2005)
Journal Article
Renau-Morata, B., Nebauer, S. G., Sales, E., Allainguillaume, J., Caligari, P., & Segura, J. (2005). Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA. American Journal of Botany, 92(5), 875-884. https://doi.org/10.3732/ajb.92.5.875

Cedrus atlantica (Pinaceae) is a large and exceptionally long-lived conifer native to the Rif and Atlas Mountains of North Africa. To assess levels and patterns of genetic diversity of this species, samples were obtained throughout the natural range... Read More about Genetic diversity and structure of natural and managed populations of Cedrus atlantica (Pinaceae) assessed using random amplified polymorphic DNA.

The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model (2004)
Journal Article
Elliott, L. J., Mason, D. C., Wilkinson, M. J., Allainguillaume, J., Norris, C., Alexander, M., & Welters, R. (2004). The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model. Journal of Applied Ecology, 41(6), 1174-1184. https://doi.org/10.1111/j.0021-8901.2004.00963.x

1. There is concern over the possibility of unwanted environmental change following transgene movement from genetically modified (GM) rapeseed Brassica napus to its wild and weedy relatives. 2. The aim of this research was to develop a remote sensing... Read More about The role of satellite image-processing for national-scale estimates of gene flow from genetically modified crops: Rapeseed in the UK as a model.

Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom (2003)
Journal Article
Shaw, M. W., Wilkinson, M. J., Elliott, L. J., Allainguillaume, J., Norris, C., Welters, R., …Mason, D. C. (2003). Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom. Science, 302(5644), 457-459. https://doi.org/10.1126/science.1088200

Measures blocking hybridization would prevent or reduce biotic or environmental change caused by gene flow from genetically modified (GM) crops to wild relatives. The efficacy of any such measure depends on hybrid numbers within the legislative regio... Read More about Hybridization Between Brassica napus and B. rapa on a National Scale in the United Kingdom.

A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors (2000)
Journal Article
Wilkinson, M. J., Davenport, I. J., Charters, Y. M., Jones, A. E., Allainguillaume, J., Butler, H. T., …Raybould, A. F. (2000). A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors. Molecular Ecology, 9(7), 983-991. https://doi.org/10.1046/j.1365-294X.2000.00986.x

One of the major environmental concerns over genetically modified (GM) crops relates to transgene movement into wild relatives. The pattern of hybridization ultimately affects the scale and rapidity of ecological change and the feasibility of contain... Read More about A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors.

Quantifying gene movement from oilseed rape to its wild relatives using remote sensing (2000)
Journal Article
Davenport, I. J., Wilkinson, M. J., Mason, D. C., Charters, Y. M., Jones, A. E., Allainguillaume, J., …Raybould, A. F. (2000). Quantifying gene movement from oilseed rape to its wild relatives using remote sensing. International Journal of Remote Sensing, 21(18), 3567-3573. https://doi.org/10.1080/014311600750037598

The potential environmental risks due to gene flow from genetically modified (GM) crops into wild plant populations are currently of great concern. Of particular interest is gene flow from GM oilseed rape (Brassica napus) into its wild parental speci... Read More about Quantifying gene movement from oilseed rape to its wild relatives using remote sensing.

Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild (1999)
Journal Article
Maunder, M., Culham, A., Bordeu, A., Allainguillaume, J., & Wilkinson, M. J. (1999). Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild. Molecular Ecology, 8(5), 725-738

Sophora toromiro was endemic to the Pacific island of Papa Nui (Easter Island) and is extinct in the wild. The species has survived in private and botanic gardens. The species is the subject of an international study to support its conservation ex si... Read More about Genetic diversity and pedigree for Sophora toromiro (Leguminosae): A tree extinct in the wild.

Evidence that genes from the male parent may influence the morphology of potato dihaploids (1997)
Journal Article
Barr, S. N., Allainguillaume, J., Wilkinson, M. J., Clulow, S. A., & Barr, S. N. R. (1997). Evidence that genes from the male parent may influence the morphology of potato dihaploids. TAG Theoretical and Applied Genetics, 94(2), 241-248. https://doi.org/10.1007/s001220050406

A number of recent studies have provided evidence that potato dihaploids (S. tuberosum) contain and express DNA front the male (dihaploid inducer) parent, S. phureja. The importance of this for breeding programmes that use dihaploid potatoes is to so... Read More about Evidence that genes from the male parent may influence the morphology of potato dihaploids.

Evidence for somatic translocation during potato dihaploid induction (1995)
Journal Article
Wilkinson, M. J., Bennett, S. T., Clulow, S. A., Allainguillaume, J., Harding, K., & Bennett, M. D. (1995). Evidence for somatic translocation during potato dihaploid induction. Heredity, 74(2), 146-151. https://doi.org/10.1038/hdy.1995.21

Potato dihaploid PDH55 (Solatium tuberosum) is exclusively euploid (2n = 24) but apparently contains and expresses DNA from dihaploid inducer IVP48 (S. phureja). Genomic in situ hybridization (GISH) suggested IVP48 DNA incorporated stably into PDH55... Read More about Evidence for somatic translocation during potato dihaploid induction.