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Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK

Kile, Helene; Arnold, Dawn; Allainguillaume, Joel; Denman, Sandra; Brady, Carrie

Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK Thumbnail


Authors

Helene Kile

Dawn Arnold

Sandra Denman

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Carrie Brady Carrie.Brady@uwe.ac.uk
Senior Lecturer - CHSS - SOAS



Abstract

Several strains of a previously undescribed bacterial species were isolated from mature Tilia hybrid trees suffering from bleed-ing cankers at various geographic locations in the UK. The strains were Gram-negative, facultatively anaerobic, and partial sequencing of the gyrB gene revealed that the strains belong to the genus Brenneria with the closest phylogenetic neighbours being Brenneria corticis and Brenneria nigrifluens. Further investigation using a polyphasic approach was undertaken to deter-mine the taxonomic position of the novel species. Phylogenies based on the 16S rRNA gene and multilocus sequence analysis of partial housekeeping gene sequences of gyrB, rpoB, infB and atpD revealed that the strains formed an independent cluster within the genus Brenneria. The phenotypic and chemotaxonomic assays demonstrated that the strains could be differentiated from the closest relatives. Genome analysis of representative strains revealed in silico DNA–DNA hybridization values below the threshold for species delimitation, although the average nucleotide identity values obtained when compared to B. corticis (95.9–96%) were slightly higher than the suggested cut-off value of 95%. However, as all other data suggests that the strains belong to a novel taxon that can be differentiated from the closest relatives, we propose that the strains represent a novel species in the genus Brenneria, Brenneria tiliae sp. nov. (type strain WC1b.1T=LMG 32575T=NCPPB 4697T).

Citation

Kile, H., Arnold, D., Allainguillaume, J., Denman, S., & Brady, C. (2022). Brenneria tiliae sp. nov., isolated from symptomatic Tilia × moltkei and Tilia × europaea trees in the UK. International Journal of Systematic and Evolutionary Microbiology, 72(10), https://doi.org/10.1099/ijsem.0.005515

Journal Article Type Article
Acceptance Date Jun 29, 2022
Online Publication Date Oct 7, 2022
Publication Date Oct 7, 2022
Deposit Date Jun 30, 2022
Publicly Available Date Mar 29, 2024
Journal International Journal of Systematic and Evolutionary Microbiology
Print ISSN 1466-5026
Electronic ISSN 1466-5034
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 72
Issue 10
DOI https://doi.org/10.1099/ijsem.0.005515
Keywords Brenneria tiliae sp. nov.; Tilia x moltkei; Tilia x europaea; trees; UK trees, Brenneria , Brenneria tiliae , Enterobacterales , lime , taxonomy and Tilia
Public URL https://uwe-repository.worktribe.com/output/9665727
Publisher URL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005515

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Description of Brenneria tiliae sp. nov. isolated from symptomatic Tilia x moltkei and Tilia x europaea trees in the UK (2.8 Mb)
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Licence
http://creativecommons.org/licenses/by/4.0/

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
'© Brady, C., Arnold, D., Allainguillaume, J., Denman, S., & Kile, H, 2022. The definitive peer reviewed, edited version of this article is published in
International Journal of Systemic and Evolutionary Biology, Volume 72, Issue 10, 2022. https://doi.org/10.1099/ijsem.0.005515




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