Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK

Bueno-Gonzalez, Victoria; Brady, Carrie; Denman, Sandra; Plummer, Sarah; Allainguillaume, Joel; Arnold, Dawn

Abstract

Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the strains in two separate phylogenetic clusters in the Pseudomonas straminea group, with Pseudomonas flavescens as the closest phylogenetic relative. Multilocus sequence analyses of the gyrB, rpoD and rpoB genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and in silico DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel Pseudomonas species, Pseudomonasdaroniae sp. nov. (type strain FRB 228T=LMG 31087T=NCPPB 4672T) and Pseudomonasdryadis sp. nov. (type strain FRB 230T=LMG 31087T=NCPPB 4673T).

Citation

Bueno-Gonzalez, V., Brady, C., Denman, S., Plummer, S., Allainguillaume, J., & Arnold, D. (2019). Pseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK. International Journal of Systematic and Evolutionary Microbiology, 69(11), 3368-3376. https://doi.org/10.1099/ijsem.0.003615

• Journal Article Type: Article
• Acceptance Date: Jul 8, 2019
• Online Publication Date: Nov 1, 2019
• Publication Date: Nov 1, 2019
• Deposit Date: Aug 21, 2019
• Publicly Available Date: Aug 22, 2019
• Journal: International Journal of Systematic and Evolutionary Microbiology
• Print ISSN: 1466-5026
• Electronic ISSN: 1466-5034
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 69
• Issue: 11
• Pages: 3368-3376
• DOI: https://doi.org/10.1099/ijsem.0.003615
• Keywords: Ecology, Evolution, Behavior and Systematics; Microbiology; General Medicine; Pseudomonas daroniae; Pseudomonas dryadis; Taxonomy; acute oak decline
• Public URL: https://uwe-repository.worktribe.com/output/2377419