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The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges

Djawad, Yasser Abd; Attwood, David; Kiely, Janice; Luxton, Richard

The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges Thumbnail


Authors

Yasser Abd Djawad

David Attwood David.Attwood@uwe.ac.uk
Associate Professor in Biosensing Systems

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

© 2019 In this paper we present a non-contact, impedance-based sensor system capable of characterizing the toxic response of cells to three different types of toxin. ECV304 cells were treated with 1 mM Hydrogen peroxide, 5% Dimethyl Sulfoxide, and 10 μg/ml saponin. Impedance spectroscopy was performed over a 2 h period on the cells within a commercial cell growth chamber, positioned on a pair of measurement electrodes, at frequencies between 200 and 830 kHz at 10 kHz intervals. Analysis of the impedance data was undertaken using the feature-extraction technique, Detrended Fluctuation Analysis (DFA). DFA scales the autocorrelation of a non-stationary signal, such as those generated using impedance spectroscopy for cytotoxicity testing. The correlation between the average fluctuation of the signal, F(n) (and scaling exponent, α) and a measurement of the cell size from image analysis was evaluated. The results showed that F(n) and α were strongly related to the changes of the morphological size of the cells. The results demonstrated that non-contact impedance spectroscopy, coupled with DFA can be used to monitor cell size in real time.

Journal Article Type Article
Acceptance Date Feb 27, 2019
Online Publication Date Mar 1, 2019
Publication Date Apr 1, 2019
Deposit Date Apr 29, 2019
Publicly Available Date Apr 29, 2019
Journal Sensing and Bio-Sensing Research
Print ISSN 2214-1804
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 23
Article Number 100269
DOI https://doi.org/10.1016/j.sbsr.2019.100269
Keywords Cell toxicity; Impedance spectroscopy; Morphological size changes
Public URL https://uwe-repository.worktribe.com/output/849488
Publisher URL https://doi.org/10.1016/j.sbsr.2019.100269
Contract Date Apr 29, 2019

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