Skip to main content

Research Repository

Advanced Search

The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges

Djawad, Yasser Abd; Attwood, David; Kiely, Janice; Luxton, Richard

The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges Thumbnail


Authors

Yasser Abd Djawad

David Attwood David.Attwood@uwe.ac.uk
Associate Professor in Biosensing Systems

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

© 2019 In this paper we present a non-contact, impedance-based sensor system capable of characterizing the toxic response of cells to three different types of toxin. ECV304 cells were treated with 1 mM Hydrogen peroxide, 5% Dimethyl Sulfoxide, and 10 μg/ml saponin. Impedance spectroscopy was performed over a 2 h period on the cells within a commercial cell growth chamber, positioned on a pair of measurement electrodes, at frequencies between 200 and 830 kHz at 10 kHz intervals. Analysis of the impedance data was undertaken using the feature-extraction technique, Detrended Fluctuation Analysis (DFA). DFA scales the autocorrelation of a non-stationary signal, such as those generated using impedance spectroscopy for cytotoxicity testing. The correlation between the average fluctuation of the signal, F(n) (and scaling exponent, α) and a measurement of the cell size from image analysis was evaluated. The results showed that F(n) and α were strongly related to the changes of the morphological size of the cells. The results demonstrated that non-contact impedance spectroscopy, coupled with DFA can be used to monitor cell size in real time.

Citation

Djawad, Y. A., Attwood, D., Kiely, J., & Luxton, R. (2019). The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges. Sensing and Bio-Sensing Research, 23, 100269. https://doi.org/10.1016/j.sbsr.2019.100269

Journal Article Type Article
Acceptance Date Feb 27, 2019
Online Publication Date Mar 1, 2019
Publication Date Apr 1, 2019
Journal Sensing and Bio-Sensing Research
Print ISSN 2214-1804
Electronic ISSN 2214-1804
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 23
Pages 100269
DOI https://doi.org/10.1016/j.sbsr.2019.100269
Public URL https://uwe-repository.worktribe.com/output/849488
Publisher URL https://doi.org/10.1016/j.sbsr.2019.100269

Files







You might also like



Downloadable Citations