The application of detrended fluctuation analysis to assess physical characteristics of the human cell line ECV304 following toxic challenges

Djawad, Yasser Abd; Attwood, David; Kiely, Janice; Luxton, Richard

Abstract

© 2019 In this paper we present a non-contact, impedance-based sensor system capable of characterizing the toxic response of cells to three different types of toxin. ECV304 cells were treated with 1 mM Hydrogen peroxide, 5% Dimethyl Sulfoxide, and 10 μg/ml saponin. Impedance spectroscopy was performed over a 2 h period on the cells within a commercial cell growth chamber, positioned on a pair of measurement electrodes, at frequencies between 200 and 830 kHz at 10 kHz intervals. Analysis of the impedance data was undertaken using the feature-extraction technique, Detrended Fluctuation Analysis (DFA). DFA scales the autocorrelation of a non-stationary signal, such as those generated using impedance spectroscopy for cytotoxicity testing. The correlation between the average fluctuation of the signal, F(n) (and scaling exponent, α) and a measurement of the cell size from image analysis was evaluated. The results showed that F(n) and α were strongly related to the changes of the morphological size of the cells. The results demonstrated that non-contact impedance spectroscopy, coupled with DFA can be used to monitor cell size in real time.

• Journal Article Type: Article
• Acceptance Date: Feb 27, 2019
• Online Publication Date: Mar 1, 2019
• Publication Date: Apr 1, 2019
• Deposit Date: Apr 29, 2019
• Publicly Available Date: Apr 29, 2019
• Journal: Sensing and Bio-Sensing Research
• Print ISSN: 2214-1804
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Article Number: 100269
• DOI: https://doi.org/10.1016/j.sbsr.2019.100269
• Keywords: Cell toxicity; Impedance spectroscopy; Morphological size changes
• Public URL: https://uwe-repository.worktribe.com/output/849488
• Publisher URL: https://doi.org/10.1016/j.sbsr.2019.100269
• Contract Date: Apr 29, 2019