Ternary nanocomposite potentiates the lysophosphatidic acid effect on human osteoblast (MG63) maturation

Elkhenany, Hoda; Elkodous, Mohamed Abd; Mansell, Jason

Abstract

Aim: This study aimed to investigate the potential of ternary nanocomposite (TNC) to support MG63 osteoblast maturation to EB1089-(3S)1-fluoro-3-hydroxy-4-(oleoyloxy)butyl-1-phosphonate (FHBP) cotreatment. Materials & methods: Binary (P25/reduced graphene oxide [rGO]) nanocomposite was prepared, and silver (Ag) nanoparticles were loaded onto the surface to form TNC (P25/rGO/Ag). The influence of TNC on proliferation, alkaline phosphatase activity and osteogenic gene expression was evaluated in a model of osteoblast maturation wherein MG63 were costimulated with EB1089 and FHBP. Results: TNC had no cytotoxic effect on MG63. The addition of TNC to EB1089-FHBP cotreatment enhanced the maturation of MG63, as supported by the greater alkaline phosphatase activity and OPN and OCN gene expression. Conclusion: TNC could serve as a promising carrier for FHBP, opening up possibilities for its application in bone regeneration.

• Journal Article Type: Article
• Acceptance Date: Oct 10, 2023
• Online Publication Date: Oct 10, 2023
• Publication Date: Sep 30, 2023
• Deposit Date: Oct 18, 2023
• Publicly Available Date: Oct 1, 2024
• Journal: Nanomedicine
• Print ISSN: 1743-5889
• Electronic ISSN: 1748-6963
• Publisher: Future Medicine
• Peer Reviewed: Peer Reviewed
• Volume: 18
• Issue: 21
• Pages: 1459-1475
• DOI: https://doi.org/10.2217/nnm-2023-0117
• Public URL: https://uwe-repository.worktribe.com/output/11183476