Towards the development of molecularly imprinted polymer based screen-printed sensors for metabolites of PAHs

Kirsch, N.; Hart, J. P.; Bird, D. J.; Luxton, R. W.; McCalley, D. V.; Kirsch, Nicole; Hart, John P.; Bird, David J.; Luxton, Richard W.; McCalley, David V.

Abstract

This paper describes the fabrication of a sensor for 1-hydroxypyrene (1-OHP) based on a screen-printed carbon electrode (SPCE) modified with a molecularly imprinted polymer (MIP); 1-OHP was chosen as a model metabolite of polyaromatic hydrocarbons (PAHs). It was shown that 1-OHP could be readily oxidised at a plain SPCE and the electrochemical mechanism was found to involve an ECE (electron transfer-chemical reaction-electron transfer) process. The MIP for 1-OHP was prepared using only divinylbenzene (DVB) and styrene as monomers and the binding was only based on hydrophobic interactions. Batch binding studies revealed that optimum uptake of 1-OHP by the MIP occurred from solutions containing 35% water in methanol. Selectivity of the binding sites in the MIP was examined by performing uptake studies in the same solution containing either phenol or 1-naphthol; the specific binding of 1-OHP was twenty times greater than the former and five times greater than the latter. Preliminary calibration studies were performed with the MIP-SPCE using a two-step approach; accumulation was carried out in 35% water in methanol followed by measurement in 50% methanol-0.025 mol dm-3 phosphate buffer pH 12. This two-step non-competitive affinity assay gave encouraging results and indicated potential for use in pollution studies.

• Presentation Conference Type: Conference Paper (published)
• Publication Date: Dec 10, 2001
• Deposit Date: Aug 10, 2010
• Journal: Analyst
• Print ISSN: 0003-2654
• Electronic ISSN: 1364-5528
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 126
• Issue: 11
• Pages: 1936-1941
• DOI: https://doi.org/10.1039/b108008n
• Public URL: https://uwe-repository.worktribe.com/output/1091342
• Publisher URL: http://dx.doi.org/10.1039/b108008n
• Contract Date: Nov 15, 2016