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Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae

Lewis, M.; Amey, Richard; Lewis, Mervyn; Macdonald, Heather; Neill, Steven; Schleicher, T.; Spencer-Phillips, Peter T.N.; Slinn, J.

Authors

M. Lewis

Richard Amey

Mervyn Lewis mervyn.lewis@uwe.ac.uk

T. Schleicher

J. Slinn



Contributors

Ales Lebeda
Editor

B.M. Cooke
Editor

Abstract

A proteomic approach was used to identify host proteins altering in abundance during Peronospora viciae infection of a susceptible cultivar of pea (Pisum sativum cv. Livioletta). Proteins were extracted from fully developed pea leaflets at 4 days post-inoculation, before visible symptoms were apparent. Cytoplasmic proteins and membrane-and nucleic acid-associated proteins from infected and control leaves were examined using two-dimensional difference gel electrophoresis. The majority of proteins had a similar abundance in control and infected leaves; however, several proteins were altered in abundance and twelve were found to have increased significantly in the latter. These proteins were selected for either matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry or electro-spray ionisation quadrupole time-of-flight tandem mass spectrometry analysis following trypsin digestion, with sequence identity being assigned to eight of the proteins. These included the ABR17 stress-response protein, the pathogen-induced PI176 protein, three photosynthetic proteins, a glycine-rich RNA binding protein and two glyceraldehyde 3-phosphate dehydrogenases (cytosolic and chloroplastic) which can be induced by a range of abiotic and biotic stresses in many plant species. The possible roles of these proteins in the response of the pea plant during P. viciae infection are discussed. This study represents the first proteomic analysis of downy mildew infection of pea leaves, and provides the basis for further work to elucidate molecular mechanisms of compatibility in P. viciae infections. © 2008 Springer Netherlands.

Publication Date Dec 1, 2008
Journal The Downy Mildews - Genetics, Molecular Biology and Control
Peer Reviewed Peer Reviewed
Pages 41-55
Book Title The Downy Mildews - Genetics, Molecular Biology and Control
ISBN 978-1-4020-8972-5
APA6 Citation Spencer-Phillips, P. T., Neill, S. J., Macdonald, H., Lewis, M., Amey, R. C., Amey, R., …Spencer-Phillips, P. T. (2008). Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae. In A. Lebeda, P. T. Spencer-Phillips, & B. Cooke (Eds.), The Downy Mildews - Genetics, Molecular Biology and Control, (41-55). Springer Netherlands. https://doi.org/10.1007/978-1-4020-8973-2_5
DOI https://doi.org/10.1007/978-1-4020-8973-2_5
Keywords electrophoresis, DIGE, MALDI-TOF, mass spectrometry, oomycete, protein
Publisher URL http://dx.doi.org/10.1007/978-1-4020-8973-2_5