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Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae

Amey, Richard; Slinn, Jenna; Lewis, Mervyn; Macdonald, Heather; Neill, Steven; Spencer-Phillips, Peter T.N.; Schleicher, T.

Authors

Richard Amey

Jenna Slinn

Mervyn Lewis mervyn.lewis@uwe.ac.uk

T. Schleicher



Abstract

A proteomic approach was used to identify host proteins altering in abundance during Peronospora viciae infection of a susceptible cultivar of pea (Pisum sativum cv. Livioletta). Proteins were extracted from fully developed pea leaflets at 4 days post-inoculation, before visible symptoms were apparent. Cytoplasmic proteins and membrane- and nucleic acid-associated proteins from infected and control leaves were examined using two-dimensional difference gel electrophoresis. The majority of proteins had a similar abundance in control and infected leaves; however, several proteins were altered in abundance and twelve were found to have increased significantly in the latter. These proteins were selected for either matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry or electro-spray ionisation quadrupole time-of-flight tandem mass spectrometry analysis following trypsin digestion, with sequence identity being assigned to eight of the proteins. These included the ABR17 stress-response protein, the pathogen-induced PI176 protein, three photosynthetic proteins, a glycine-rich RNA binding protein and two glyceraldehyde 3-phosphate dehydrogenases (cytosolic and chloroplastic) which can be induced by a range of abiotic and biotic stresses in many plant species. The possible roles of these proteins in the response of the pea plant during P. viciae infection are discussed. This study represents the first proteomic analysis of downy mildew infection of pea leaves, and provides the basis for further work to elucidate molecular mechanisms of compatibility in P. viciae infections. © 2008 KNPV.

Journal Article Type Conference Paper
Publication Date Sep 1, 2008
Journal European Journal of Plant Pathology
Print ISSN 0929-1873
Publisher Springer (part of Springer Nature)
Peer Reviewed Peer Reviewed
Volume 122
Issue 1
Pages 41-55
APA6 Citation Spencer-Phillips, P. T., Neill, S. J., Macdonald, H., Lewis, M., Slinn, J., Amey, R. C., …Spencer-Phillips, P. T. (2008). Proteomic analysis of a compatible interaction between Pisum sativum (pea) and the downy mildew pathogen Peronospora viciae. European Journal of Plant Pathology, 122(1), 41-55. https://doi.org/10.1007/s10658-008-9313-2
DOI https://doi.org/10.1007/s10658-008-9313-2
Keywords electrophoresis, DIGE, MALDI-TOF, mass spectrometry, oomycete, protein
Publisher URL http://dx.doi.org/10.1007/s10658-008-9313-2