Prof Michael Ladomery's Outputs (62)

Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm (2007)
Journal Article

Alternative splicing, first discovered in the 1970s, has emerged as one of the key generators of proteomic diversity. Not surprisingly, alternative splicing is increasingly linked to the etiology of cancer. This is illustrated by vascular endothelial... Read More about Alternative splicing in angiogenesis: The vascular endothelial growth factor paradigm.

A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1 (2007)
Journal Article

Glomerular podocytes are essential for blood filtration in the kidney underpinned by their unique cytoskeletal morphology. An increasing number of kidney diseases are being associated with key podocyte abnormalities. The Wilms tumour suppressor gene... Read More about A proteomic investigation of glomerular podocytes from a Denys-Drash Syndrome patient with a mutation in the Wilms tumour suppressor gene WT1.

Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562 (2006)
Journal Article

The WT1 gene is a key player in acute myeloid leukaemia, in which it is frequently over-expressed. WT1 encodes a multifunctional zinc finger protein transcription factor, which also binds mRNA. Thus increasing evidence suggests that WT1 works both at... Read More about Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562.

WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo (2006)
Journal Article

Wilm's tumor protein 1 (WT1), a protein implicated in various cancers and developmental disorders, consists of two major isoforms: WT1(-KTS), a transcription factor, and WT1(+KTS), a post-transcriptional regulator that binds to RNA and can interact w... Read More about WT1 interacts with the splicing protein RBM4 and regulates its ability to modulate alternative splicing in vivo.

The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain (2006)
Journal Article

Mutations in WT1 are associated with developmental syndromes that affect the urogenital system and neoplasms, including Wilms tumour, acute myeloid leukemia, and breast and prostate cancers. The WT1 protein belongs to the early growth response family... Read More about The Wilms tumour suppressor protein WT1 (+KTS isoform) binds alpha-actinin 1 mRNA via its zinc-finger domain.

The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron (2006)
Journal Article

The Wilms' tumor 1 (WT1) gene plays an important role in mammalian urogenital development, and dysregulation of this gene is observed in many human cancers. Alternative splicing of WT1 RNA leads to the expression of two major protein isoforms, WT1(+K... Read More about The Wilms' tumor 1 (WT1) gene (+KTS isoform) functions with a CTE to enhance translation from an unspliced RNA with a retained intron.

Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation (2004)
Journal Article

Wt1 is a tumour suppressor gene, mutation of which is a cause of Wilms' tumour, a childhood renal nephroblastoma. Wt1 is expressed in a rich pattern during renal development suggesting that it acts at three stages: determination of the kidney area, t... Read More about Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation.

Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis (2003)
Journal Article

Denys-Drash syndrome (DDS) is caused by dominant mutations of the Wilms' tumour suppressor gene, WT1, and characterized by a nephropathy involving diffuse mesangial sclerosis, male pseudohermaphroditism and/ or Wilms' tumourigenesis. Previously, we r... Read More about Murine Denys-Drash syndrome: Evidence of podocyte de-differentiation and systemic mediation of glomerulosclerosis.

Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one (2003)
Journal Article

The Wilms' tumour suppressor gene WT1 encodes a protein involved in urogenital development and disease. The salient feature of WT1 is the presence of four 'Krüppel'-type C2-H2 zinc fingers in the C-terminus. Uniquely to WTI, an evolutionarily conserv... Read More about Expression in Xenopus oocytes shows that WT1 binds transcripts in vivo, with a central role for zinc finger one.

Multifunctional zinc finger proteins in development and disease (2002)
Journal Article

Post-transcriptional processes contribute significantly towards the generation of proteomic diversity. An increasing number of mutations have been described that affect genes encoding components of the post-transcriptional machinery. In particular, m... Read More about Multifunctional zinc finger proteins in development and disease.

4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus (2000)
Journal Article

The protein C4SR contains two cysteine 4 (C 4) zinc-finger motifs at its amino terminus, a stretch of acidic residues in the middle and a series of serine-arginine (SR) repeats at its carboxyl terminus. A cDNA clone encoding the zinc-finger domain wa... Read More about 4SR, a novel zinc-finger protein with SR-repeats, is expressed during early development of Xenopus.

Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein (1999)
Journal Article

The tumor suppressor gene WT1 encodes a zinc finger protein, which consists of four C-terminal C2-H2 zinc fingers of the Kruppel type, and at the N terminus a Q/P-rich trans-regulatory domain, both characteristic of transcription factors. However, re... Read More about Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)+ ribonucleoprotein.

Multiple roles for the Wilms' tumor suppressor, WT1 (1999)
Journal Article

Wilms' tumor is a childhood kidney tumor that is a striking example of the way that cancer may arise through development gone awry. A proportion of these tumors develop as a result of the loss of function mutations in the Wilms' tumor suppressor gene... Read More about Multiple roles for the Wilms' tumor suppressor, WT1.

Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes (1997)
Journal Article

Modification of core histones can alter chromatin structure, facilitating the activation and repression of genes. A key example is the acetylation of N-terminal lysines of the core histones. Recently, the mammalian histone deacetylase HD1 was cloned... Read More about Xenopus HDm, a maternally expressed histone deacetylase, belongs to an ancient family of acetyl-metabolizing enzymes.

Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes (1997)
Journal Article

Recent findings indicate that substantial cross-talk may exist between transcriptional and post-transcriptional processes. Firstly, there are suggestions that specific promoters influence the post-transcriptional fate of transcripts, pointing to comm... Read More about Multifunctional proteins suggest connections between transcriptional and post-transcriptional processes.

Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes (1997)
Journal Article

In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on the phosphoproteins pp60/56, which are Y-box proteins involved in a general packaging of mRNA. We now identify a third, abund... Read More about Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.

Masking of messenger RNA by Y-box proteins (1996)
Journal Article

The Y-box proteins are defined by their ability to bind to Y-box promoter elements and to help regulate transcription of a wide variety of genes. However, Y-box proteins are also identified as abundant proteins in the cytoplasm of germ cells, where t... Read More about Masking of messenger RNA by Y-box proteins.

Transcription and masking of mRNA in germ cells: Involvement of Y-box proteins (1996)
Journal Article

Gametogenesis is directed by various specialized genetic mechanisms which, to a considerable extent, apply to the production of both eggs and sperm and have been conserved across a wide spectrum of eukaryotic organisms. Two key aspects which are disc... Read More about Transcription and masking of mRNA in germ cells: Involvement of Y-box proteins.

A role for Y‐box proteins in cell proliferation (1995)
Journal Article

Members of the Y‐box (YB) family of transcription factors are expressed in a wide range of cell types and are implicated in the regulation of a rapidly increasing number of genes. Although the biological activities of YB proteins appear to be varied,... Read More about A role for Y‐box proteins in cell proliferation.

Binding of Y-box proteins to RNA: Involvement of different protein domains (1994)
Journal Article

Eukaryotlc Y-box proteins are reported to interact with
a wide variety of nucleic acid structures to act as
transcription factors and mRNA masking proteins. The
modular structure of Y-box proteins Includes a highly
conserved N-termlnal cold-shock... Read More about Binding of Y-box proteins to RNA: Involvement of different protein domains.