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Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562

Morrison, Avril A.; Ladomery, Michael

Authors

Avril A. Morrison



Abstract

The WT1 gene is a key player in acute myeloid leukaemia, in which it is frequently over-expressed. WT1 encodes a multifunctional zinc finger protein transcription factor, which also binds mRNA. Thus increasing evidence suggests that WT1 works both at the DNA and mRNA level, not only in the urogenital system but also in other contexts. Nuclear poly(A)+ mRNP particles were isolated by oligo(dT) chromatography from the human acute myeloid leukemia cell lines HL60 and K562, and analysed by Western blotting and 2D minigels. MALDI-TOF demonstrated the presence of hnRNP proteins, splice factors, and unexpectedly vimentin in the mRNP fraction. WT1 was also shown to be present in nuclear mRNP particles suggesting that in leukaemia, and by extension in all cancers in which it is involved, WT1 works both at the DNA and mRNA target level. © 2006 Elsevier Ireland Ltd. All rights reserved.

Citation

Morrison, A. A., & Ladomery, M. (2006). Presence of WT1 in nuclear messenger RNP particles in the human acute myeloid leukemia cell lines HL60 and K562. Cancer Letters, 244(1), 136-141. https://doi.org/10.1016/j.canlet.2005.12.018

Journal Article Type Article
Publication Date Nov 28, 2006
Journal Cancer Letters
Print ISSN 0304-3835
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 244
Issue 1
Pages 136-141
DOI https://doi.org/10.1016/j.canlet.2005.12.018
Keywords WT1, hnRNP H, mRNP proteomics, acute myeloid leukaemia, K562, HL60
Public URL https://uwe-repository.worktribe.com/output/1035391
Publisher URL http://dx.doi.org/10.1016/j.canlet.2005.12.018