Skip to main content

Research Repository

Advanced Search

Darren Reynolds' Outputs (75)

Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp. (2023)
Journal Article

Background: Di(2-ethylhexyl) phthalate (DEHP) is a widely detected plasticizer and a priority pollutant of utmost concern for its adverse impact on humans, wildlife and the environment. To eliminate such toxic burden, biological processes are the mos... Read More about Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp..

Clean water for all (2022)
Digital Artefact

The World Health Organization (WHO) and the United Nations Children’s Fund (UNICEF) estimated that, as of 2020, 2 billion people across the globe did not have access to safely managed drinking water.

Ideally, safely managed drinking water should b... Read More about Clean water for all.

Managing potential pathogens in stored rainwater using small-scale in-situ electrochemical activation (2022)
Presentation / Conference Contribution

Rainwater can be a lifeline for many people who do not have piped water supplies, experience dry seasons, or live-in fresh water scarce areas. The quality of stored rainwater can deteriorate over time, due to increasing concentrations of contaminants... Read More about Managing potential pathogens in stored rainwater using small-scale in-situ electrochemical activation.

A case study: The deployment of a novel in situ fluorimeter for monitoring biological contamination within the urban surface waters of Kolkata, India (2022)
Journal Article

The quality and health of many of our vital freshwater systems are poor. To tackle this with ever increasing pressures from anthropogenic and climatic changes, we must improve water quality monitoring and devise and implement more appropriate water q... Read More about A case study: The deployment of a novel in situ fluorimeter for monitoring biological contamination within the urban surface waters of Kolkata, India.

The in situ production of aquatic fluorescent organic matter in a simulated freshwater laboratory model (2022)
Journal Article

Dissolved organic matter (DOM) is ubiquitous throughout aquatic systems. Fluorescence techniques can be used to characterize the fluorescing proportion of DOM, aquatic fluorescent organic matter (AFOM). AFOM is conventionally named in association wit... Read More about The in situ production of aquatic fluorescent organic matter in a simulated freshwater laboratory model.

The efficacy of chlorine-based disinfectants against planktonic and biofilm bacteria for decentralised point-of-use drinking water (2021)
Journal Article

Chlorine solutions are used extensively for the production of biologically safe drinking water. The capability of point-of-use [POU] drinking water treatment systems has gained interest in locations where centralised treatment systems and distributio... Read More about The efficacy of chlorine-based disinfectants against planktonic and biofilm bacteria for decentralised point-of-use drinking water.

Real-time detection of volatile metabolites enabling species-level discrimination of bacterial biofilms associated with wound infection (2021)
Journal Article

Aims: The main aim of this study was to investigate the real-time detection of volatile metabolites for the species-level discrimination of pathogens associated with clinically relevant wound infection, when grown in a collagen wound biofilm model. M... Read More about Real-time detection of volatile metabolites enabling species-level discrimination of bacterial biofilms associated with wound infection.

Laboratory in-situ production of autochthonous and allochthonous fluorescent organic matter by freshwater bacteria (2021)
Journal Article

This work investigates the origin and range of fluorescent organic matter (FOM) produced in-situ by environmentally sourced freshwater bacteria. Aquatic FOM is an essential component in global carbon cycling and is generally classified as either auto... Read More about Laboratory in-situ production of autochthonous and allochthonous fluorescent organic matter by freshwater bacteria.

Sediment and surface water microplastic contamination along the Ganges River, from the Himalayan foothills to the tidal reach downstream of Kolkata, India (2021)
Presentation / Conference Contribution

Twenty rivers have been estimated previously to account for almost 70% of global plastic emissions to the marine environment. Of these, the Ganges River was second only to the Yangtze River. Yet, scale and complexity of the Ganges River network make... Read More about Sediment and surface water microplastic contamination along the Ganges River, from the Himalayan foothills to the tidal reach downstream of Kolkata, India.

A case study: The deployment of a novel in situ fluorimeter for monitoring biological contamination within the urban surface waters of Kolkata, India (2021)
Presentation / Conference Contribution

With increasing pressures on water resources due to population, industrialization, agriculture, urbanization and climatic changes, improved temporal and spatial understanding of water quality is required. The development of new monitoring parameters,... Read More about A case study: The deployment of a novel in situ fluorimeter for monitoring biological contamination within the urban surface waters of Kolkata, India.

Water quality across the River Ganga basin in India: Trends, dominant geochemical processes and impacts (2021)
Presentation / Conference Contribution

In a basin-wide survey of the River Ganga and key tributaries, from the Himalayan source to the Bay of Bengal in India, we aim to improve the conceptual understanding of downstream water quality trends along > 2000 km. Here we explore the spatial di... Read More about Water quality across the River Ganga basin in India: Trends, dominant geochemical processes and impacts.

Using in-situ sensors to quantify spatial variability in nutrient concentrations across the Ganges river basin (2021)
Presentation / Conference Contribution

There is increasing interest in monitoring spatial variability in biogeochemical processes using field deployable sensors. Despite this, rigorous assessments of accuracy and optimal sensor configurations remain limited for such applications. We under... Read More about Using in-situ sensors to quantify spatial variability in nutrient concentrations across the Ganges river basin.

Taking the pulse of Mother Ganga - Revealing the visible and invisible water pollution crisis along the Ganges River (2021)
Presentation / Conference Contribution

It is probably hard to overestimate the significance of the River Ganges for its spiritual, cultural and religious importance. As the worlds’ most populated river basin and a major water resource for the 400 million people inhabiting its catchment, t... Read More about Taking the pulse of Mother Ganga - Revealing the visible and invisible water pollution crisis along the Ganges River.

An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics (2019)
Journal Article

Background
The majority of in vitro studies of medically relevant biofilms involve the development of biofilm on an inanimate solid surface. However, infection in vivo consists of biofilm growth on, or suspended within, the semi-solid matrix of the... Read More about An in vitro collagen perfusion wound biofilm model; with applications for antimicrobial studies and microbial metabolomics.

Comparison of trihalomethane formation using chlorine-based disinfectants within a model system; Applications within point-of-use drinking water treatment (2019)
Journal Article

© 2019 Clayton, Thorn and Reynolds. Point-of-use (POU) drinking water treatment systems provide solutions for communities where centralized facilities are unavailable. Effective POU systems treat and reduce the number of pathogens in POU water suppl... Read More about Comparison of trihalomethane formation using chlorine-based disinfectants within a model system; Applications within point-of-use drinking water treatment.

Microbial processing and production of aquatic fluorescent organic matter in a model freshwater system (2018)
Journal Article

© 2018 by the authors. Organic matter (OM) has an essential biogeochemical influence along the hydrological continuum and within aquatic ecosystems. Organic matter derived via microbial processes was investigated within a range of model freshwater sa... Read More about Microbial processing and production of aquatic fluorescent organic matter in a model freshwater system.

CPX-351 exhibits hENT-independent uptake and can be potentiated by fludarabine in leukaemic cells lines and primary refractory AML (2018)
Journal Article

© 2018 Elsevier Ltd CPX-351, a liposomal formulation co-encapsulating cytarabine and daunorubicin (DNR) in a synergistic 5:1 M ratio, has shown favourable response in newly diagnosed elderly high-risk AML. This study assessed intracellular ara-CTP le... Read More about CPX-351 exhibits hENT-independent uptake and can be potentiated by fludarabine in leukaemic cells lines and primary refractory AML.

Development of a novel off-grid drinking water production system integrating electrochemically activated solutions and ultrafiltration membranes (2017)
Journal Article

© 2017 Elsevier Ltd Approximately 800 million people live without clean drinking water. Diarrhoea is responsible for between 1.7 and 2 million deaths each year (primarily children) which are the result of poor drinking water quality and sanitation. T... Read More about Development of a novel off-grid drinking water production system integrating electrochemically activated solutions and ultrafiltration membranes.

Assessing the antimicrobial potential of aerosolised electrochemically activated solutions (ECAS) for reducing the microbial bio-burden on fresh food produce held under cooled or cold storage conditions (2017)
Journal Article

© 2017 The main aim of this study was to assess the antimicrobial efficacy of electrochemically activated fog (ECAF) for reducing the microbial bio-burden on artificially inoculated fresh produce held under cooled (cucumber and vine tomatoes) or cold... Read More about Assessing the antimicrobial potential of aerosolised electrochemically activated solutions (ECAS) for reducing the microbial bio-burden on fresh food produce held under cooled or cold storage conditions.

In vitro discrimination of wound-associated bacteria by volatile compound profiling using selected ion flow tube-mass spectrometry (2017)
Journal Article

© 2017 The Society for Applied Microbiology Aims: To determine if bacterial species responsible for clinically relevant wound infection produce specific volatile profiles that would allow their speciation. Methods and Results: Selected ion flow tube-... Read More about In vitro discrimination of wound-associated bacteria by volatile compound profiling using selected ion flow tube-mass spectrometry.

Dissolved organic carbon transformations and microbial community response to variations in recharge waters in a shallow carbonate aquifer (2016)
Journal Article

© 2016, The Author(s). In carbonate aquifers, dissolved organic carbon from the surface drives heterotrophic metabolism, generating CO2 in the subsurface. Although this has been a proposed mechanism for enhanced dissolution at the water table, respir... Read More about Dissolved organic carbon transformations and microbial community response to variations in recharge waters in a shallow carbonate aquifer.

Characterisation of dissolved organic matter fluorescence properties by PARAFAC analysis and thermal quenching (2014)
Journal Article

The fluorescence intensity of dissolved organic matter (DOM) in aqueous samples is known to be highly influenced by temperature. Although several studies have demonstrated the effect of thermal quenching on the fluorescence of DOM, no research has be... Read More about Characterisation of dissolved organic matter fluorescence properties by PARAFAC analysis and thermal quenching.

Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay (2013)
Journal Article

The main aim of this study was to develop a standardized experimental assay to enable differential antimicrobial comparisons of test biocidal aerosols. This study represents the first chlorine-matched comparative assessment of the antimicrobial activ... Read More about Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay.

CoreGenes3.5: A webserver for the determination of core genes from sets of viral and small bacterial genomes (2013)
Journal Article

Background: CoreGenes3.5 is a webserver that determines sets of core genes from viral and small bacterial genomes as an automated batch process. Previous versions of CoreGenes have been used to classify bacteriophage genomes and mine data from pathog... Read More about CoreGenes3.5: A webserver for the determination of core genes from sets of viral and small bacterial genomes.

Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor (2013)
Presentation / Conference Contribution

Cytarabine (ara-C) is an analogue of deoxycytidine with an established role in treating AML. Cytarabine depends on the human equilibrative nucleoside transporter-1 (hENT-1) for cellular entry (1). Loss of hENT-1 function in vitro has been shown to re... Read More about Detection of intracellular ara-C/ara-CTP following treatment with the new anti-leukemic drug elacytarabine using a bioluminescent bacterial biosensor.

The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions (2013)
Journal Article

Electrochemically activated solutions (ECAS) are generated by electrolysis of NaCl solutions, and demonstrate broad spectrum antimicrobial activity and high environmental compatibility. The biocidal efficacy of ECAS at the point of production is wide... Read More about The effect of long-term storage on the physiochemical and bactericidal properties of electrochemically activated solutions.

Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments (2012)
Journal Article

Due to the limitations associated with the use of existing biocidal agents, there is a need to explore new methods of disinfection to help maintain effective bioburden control, especially within the healthcare environment. The transformation of low m... Read More about Electrochemically activated solutions: Evidence for antimicrobial efficacy and applications in healthcare environments.

Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro (2011)
Journal Article

Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro... Read More about Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro (2011)
Journal Article

Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro... Read More about Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores (2010)
Journal Article

Aims: Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species,... Read More about Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores.

Real-time monitoring of river water quality using in-line continuous acquisition of fluorescence excitation and emission matrices (2009)
Presentation / Conference Contribution

Birmingham, UK. River water was diverted to a portable laboratory via a continuous flow pump and filter system. Fluorescence excitation-emission matrices data was recorded every 3 minutes using a flow cell (1cm pathlength) coupled to a fiber optic pr... Read More about Real-time monitoring of river water quality using in-line continuous acquisition of fluorescence excitation and emission matrices.

Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration (2009)
Journal Article

The effects of photodegradation and biodegradation upon aquatic organic matter lability have been extensively researched in all aquatic systems because of the impact of these. processes upon carbon cycling, with most studies undertaken on the dissolv... Read More about Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration.

Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England (2008)
Journal Article

The fluorescence intensities of tryptophan-like, tyrosine-like and humic-like materials were determined using excitation-emission-matrices (EEMs) for a wide range of samples including natural surface waters, sewage and industrial effluents and waters... Read More about Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England.

The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy (2002)
Journal Article

The chemical and biochemical oxygen demand values of a number of synthetic and wastewater samples were determined using fluorescence spectroscopy. Treated and untreated sewage samples were obtained from a local sewage treatment works while synthetic... Read More about The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy.

Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma
Presentation / Conference Contribution

Introduction:
Maintenance of a clean, uncontaminated healthcare environment is instrumental in the delivery of modern healthcare. Nonthermal plasma is a technology with potential applications for sterilisation and disinfection in biological and medi... Read More about Low light optical detection of whole-cell bioluminescent bacterial bioreporters for visualisation of surface decontamination using nonthermal plasma.

Biofilm management; Investigating the inhibitory effects of chlorine-based disinfectants on biofilms
Presentation / Conference Contribution

Summary of key findings
Direct disinfection of established Pseudomonas aeruginosa biofilms by electrochemically activated solutions [ECAS] exhibited significantly greater anti-biofilm activity compared to NaOCl and HOCl at free chlorine concentratio... Read More about Biofilm management; Investigating the inhibitory effects of chlorine-based disinfectants on biofilms.