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Outputs (37)

An exploration of the direct biological targets of molecular hydrogen (2024)
Book Chapter
Hancock, J., May, J. E., LeBaron, T. W., Punampalam, R., & Russell, G. (2024). An exploration of the direct biological targets of molecular hydrogen. In J. Slezak, & B. Kura (Eds.), Molecular Hydrogen in Health and Disease (21-38). Springer. https://doi.org/10.1007/978-3-031-47375-3

Molecular hydrogen (H2), supplied either as a gas or in a solution, has been gaining popularity as a treatment for a variety of conditions and diseases. For example, it has been suggested to be beneficial for neurodegenerative diseases, to ease the i... Read More about An exploration of the direct biological targets of molecular hydrogen.

An interplay of gases: oxygen and hydrogen in biological systems (2024)
Journal Article
Russell, G., May, J., & Hancock, J. T. (2024). An interplay of gases: oxygen and hydrogen in biological systems. Oxygen, 4(1), 37-52. https://doi.org/10.3390/oxygen4010003

Produced by photosynthesis, oxygen (O2) is a fundamentally important gas in biological systems, playing roles as a terminal electron receptor in respiration and in host defence through the creation of reactive oxygen species (ROS). Hydrogen (H2) play... Read More about An interplay of gases: oxygen and hydrogen in biological systems.

Molecular hydrogen as a beneficial postharvest treatment (2023)
Book Chapter
Hancock, J. T., LeBaron, T. W., May, J., Thomas, A., Zulfiqar, F., & Russell, G. (2024). Molecular hydrogen as a beneficial postharvest treatment. In V. Ziogas, & F. J. Corpas (Eds.), Oxygen, Nitrogen and Sulfur Species in Post-Harvest Physiology of Horticultural Crops (303-318). Elsevier. https://doi.org/10.1016/B978-0-323-91798-8.00011-4

Molecular hydrogen (H2) has been shown to have significant effects in plants, especially during stress responses. H2 treatment can be used as a gas or as an H2-enriched solution, often termed hydrogen-rich water (HRW). Cellular effects of H2 are ofte... Read More about Molecular hydrogen as a beneficial postharvest treatment.

Gamification in biomedical science education: The successful implementation of Resimion, a scenario-based learning tool (2023)
Journal Article
May, J. E., Anderson, E., Clark, D., & Hull, J. (2023). Gamification in biomedical science education: The successful implementation of Resimion, a scenario-based learning tool. British Journal of Biomedical Science, 80, Article 11756. https://doi.org/10.3389/bjbs.2023.11756

Introduction: Scenario-based learning and gamification have many advantages in comparison to traditional didactic teaching methods, including development of many higher-level skills such as analysis and evaluation. It is hoped that these simulations... Read More about Gamification in biomedical science education: The successful implementation of Resimion, a scenario-based learning tool.

Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily (2022)
Journal Article
Hancock, J. T., Russell, G., Craig, T. J., May, J., Morse, H. R., & Stamler, J. S. (2022). Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily. Oxygen, 2(4), 578-590. https://doi.org/10.3390/oxygen2040038

Hydrogen gas (molecular hydrogen, H2) has significant effects in a range of organisms, from plants to humans. Many inert gases have been reported to have similar effects, and such responses may be most pronounced when cells are stressed. Xenon (Xe),... Read More about Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily.

Development of a paper-based lateral flow Prothrombin assay (2022)
Journal Article
Saidykhan, J., Pointon, L., Cinti, S., May, J., & Killard, A. (2022). Development of a paper-based lateral flow Prothrombin assay. Analytical Methods, 14, 3718-3726. https://doi.org/10.1039/d2ay00965j

Disorders of haemostasis result in both excessive bleeding and clotting and are a major global cause of morbidity and mortality, particularly in the developing world. A small number of simple tests can be used to screen and monitor for such dysfuncti... Read More about Development of a paper-based lateral flow Prothrombin assay.

Molecular hydrogen: Is this a viable new treatment for plants in the UK? (2021)
Journal Article
Hancock, J. T., LeBaron, T. W., May, J., Thomas, A., & Russell, G. (2021). Molecular hydrogen: Is this a viable new treatment for plants in the UK?. Plants, 10(11), Article 2270

Despite being trialed in other regions of the world, the use of molecular hydrogen (H2) for enhanced plant growth and postharvest storage of crops has yet to be widely accepted in the UK. The evidence continues to grow that treatment of plants and pl... Read More about Molecular hydrogen: Is this a viable new treatment for plants in the UK?.

Paper-based lateral flow device for the sustainable measurement of human plasma fibrinogen in low-resource settings (2021)
Journal Article
Saidykhan, J., Selevic, L., Cinti, S., May, J., & Killard, A. (2021). Paper-based lateral flow device for the sustainable measurement of human plasma fibrinogen in low-resource settings. Analytical Chemistry, 93(41), 14007-14013. https://doi.org/10.1021/acs.analchem.1c03665

Fibrinogen concentration is a major determinant of both clotting and bleeding risk. Clotting and bleeding disorders cause extensive morbidity and mortality, particularly in resource-poor and emergency settings. This is exacerbated by a lack of timely... Read More about Paper-based lateral flow device for the sustainable measurement of human plasma fibrinogen in low-resource settings.

Primary mesenchymal stromal cells in co-culture with leukaemic HL-60 cells are sensitised to cytarabine-induced genotoxicity, while leukaemic cells are protected (2021)
Journal Article
Gynn, L. E., Anderson, E., Robinson, G., Wexler, S. A., Upstill-Goddard, G., Cox, C., & May, J. E. (2021). Primary mesenchymal stromal cells in co-culture with leukaemic HL-60 cells are sensitised to cytarabine-induced genotoxicity, while leukaemic cells are protected. Mutagenesis, 36(6), 419-428. https://doi.org/10.1093/mutage/geab033

Tumour microenvironments are hallmarked in many cancer types. In haematological malignancies, bone marrow (BM) mesenchymal stromal cells (MSC) protect malignant cells from drug-induced cytotoxicity. However, less is known about malignant impact on su... Read More about Primary mesenchymal stromal cells in co-culture with leukaemic HL-60 cells are sensitised to cytarabine-induced genotoxicity, while leukaemic cells are protected.

Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells (2021)
Journal Article
Gynn, L., Lamb-Riddell, K., Cox, T., Hansen, M., Conway, M., & May, J. (2021). Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells. British Journal of Haematology, 193(S1), 46-47

Mesenchymal stromal cells (MSC) protect leukaemic cells from drug-induced toxicity within the bone marrow niche, with increasing evidence of leukaemic impact on supportive stroma. The nucleoside analogue, cytarabine (ara-C), is a front-line agent for... Read More about Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells.

Use of machine learning-enabled scenario-based teaching in Haematology and Biomedical Science, as a replacement and supplement to traditional tools and in-person teaching during COVID-19 (2021)
Journal Article
May, J., Anderson, E., Clark, D., & Hull, J. (2021). Use of machine learning-enabled scenario-based teaching in Haematology and Biomedical Science, as a replacement and supplement to traditional tools and in-person teaching during COVID-19. British Journal of Haematology, 193(S1), 72

One of the biggest challenges during the COVID-19 pandemic has been maintaining student engagement and providing the best learning experience with limited face-to-face (FTF) contact, and social distancing requirements. Whilst multiple tools are avail... Read More about Use of machine learning-enabled scenario-based teaching in Haematology and Biomedical Science, as a replacement and supplement to traditional tools and in-person teaching during COVID-19.

Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease (2019)
Journal Article
May, J., Gynn, L., Anderson, E., & Robinson, G. (2019). Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease. Mutagenesis, 34(5/6), e5. https://doi.org/10.1093/mutage/gez025

Resistance to therapy remains one of the greatest challenges in the management of the blood and bone marrow (BM) disorder, acute myeloid leukaemia (AML). This study aimed to investigate how leukaemic and BM stromal cells contribute to altered chemo-s... Read More about Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease.

Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). (2019)
Journal Article
Gynn, L. E., Anderson, E., Robinson, G. M., Wexler, S. A., Upstill-Goddard, G., Cox, C., …Morgan, R. G. (2019). Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). Blood, 134(Supplement 1), 2492. https://doi.org/10.1182/blood-2019-122891

Mesenchymal stromal cells (MSC) are known to protect leukemic cells from drug-induced toxicity within the bone marrow (BM) niche, however, less is known about leukemic impact on supportive MSC. The nucleoside-analogue, cytarabine (Ara-C), is a front-... Read More about Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML)..

Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications (2018)
Journal Article
May, J. E., Donaldson, C., Gynn, L., & Ruth Morse, H. (2018). Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications. Mutagenesis, 33(3), 241-251. https://doi.org/10.1093/mutage/gey014

© The Author(s) 2018. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. Mesenchymal stem/stromal cells (MSCs) within the bone marrow (BM) are vitally important in forming the micro-environment supporting haematop... Read More about Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications.

Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia (2017)
Journal Article
May, J., Steven, J., Taylor, A., Dean, S., & Anderson, E. (2017). Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia. Mutagenesis, 32(6), 627. https://doi.org/10.1093/mutage/gex033

Treatment success in leukaemia is impacted by patient resistance to chemotherapy including cytarabine (ara-C) and the anthracycline daunorubicin (DNR) requiring rapid pre-screening testing. DNR is converted to daunorubicinol (DNRol) by carbonyl reduc... Read More about Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia.

Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor (2017)
Journal Article
Gynn, L., May, J., Anderson, E., & Robinson, G. (2017). Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor. Mutagenesis, 32(6), 619. https://doi.org/10.1093/mutage/gex033

This study aimed to develop a 2D leukaemia-stroma model of the bone marrow for analysis of stromal-mediated chemo-protection; known to contribute to chemotherapy resistance. The model must facilitate integration of a bioluminescent biosensor HA1 for... Read More about Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor.

Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS) (2017)
Journal Article
May, J., Robinson, G., Thorn, R., & Reynolds, D. (2017). Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS). Mutagenesis, 32(6), 622-623. https://doi.org/10.1093/mutage/gex033

Electrochemically activated solutions (ECAS) are of interest as potential disinfectants due to high biocidal activity against many microorganisms. They are widely used clinically in some countries (decontamination of surgical instruments and wounds),... Read More about Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS).

Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner (2015)
Journal Article
Andrews, S., Morse, H. R., & May, J. E. (2015). Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner. Mutagenesis, 30(6), 867-868. https://doi.org/10.1093/mutage/gev074

Multiple myeloma (MM) is an incurable haematological malignancy with most patients eventually developing ad- vanced, relapsing disease and drug resistance (1,2). This may result from protection by BM mesenchymal cells (BM— MSC) interacting with MM ce... Read More about Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner.

Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure. Mutagenesis, 30(6), 874. https://doi.org/10.1093/mutage/gev074

Although gene expression analysis using real-time quan- titative reverse transcription-polymerase chain reaction (RT-qPCR) is an accurate method to evaluate cell activi- ties and phenotypes, the reliability of the analysis depends on the selection of... Read More about Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure.

The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model. Mutagenesis, 30(6), 861. https://doi.org/10.1093/mutage/gev074

Developing a novel three-dimensional (3D) in vitro model of the bone marrow microenvironment using bone mar- row mesenchymal stem cells (BM-MSCs) in commercial matrices involves a series of distinct processes. This starts with selecting an appropriat... Read More about The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model.

A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells (2015)
Journal Article
May, J. E., Allainguillaume, J., Page, K., Stratford-Smith, C., Aruomaren, A., Alloush, H., …Anderson, E. (2015). A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells. Mutagenesis, 30(6), 856. https://doi.org/10.1093/mutage/gev074

Decitabine (DAC) and azacytidine (AZA) are cytidine ana- logues and hypomethylating agents used in the manage- ment of patients with myelodysplastic syndrome (MDS), where the main goal is to prevent transformation to acute myeloid leukaemia (AML), wh... Read More about A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells.

Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment (2013)
Journal Article
May, J. E., Morse, H. R., Page, K., Soh, M., Wexler, S., Cox, C., …Donaldson, C. (2014). Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment. Mutagenesis, 29(1), 79. https://doi.org/10.1093/mutage/get060

The bone marrow (BM) microenvironment is largely com- prised of mesenchymal stromal cells (MSCs) and is vital in supporting haematopoiesis. Limited studies have indicated that the BM microenvironment is damaged by chemotherapy; however the extent and... Read More about Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment.

Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy (2013)
Journal Article
Page, K., Anderson, E., Smith, A., Reid, C., Salisbury, V., Donaldson, C., & May, J. E. (2014). Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy. Mutagenesis, 29(1), 92. https://doi.org/10.1093/mutage/get060

Cytosine arabinoside (ara-C) is a key chemotherapy drug used to treat acute myeloid leukaemia (AML), however, ap- proximately 30% of patients do not respond to this therapy. 92 An evaluative assay to determine resistance/sensitivity to ara- C prior... Read More about Effects of bone marrow stromal cells on sensitivity of leukaemic cells to chemotherapy.

Use of whole blood for analysis of disease-associated biomarkers (2013)
Journal Article
McLeod, J., Pemberton, R. M., May, J. E., Pemberton, R., Hart, J. P., McLeod, J. D., …Doran, O. (2013). Use of whole blood for analysis of disease-associated biomarkers. Analytical Biochemistry, 437(1), 59-61. https://doi.org/10.1016/j.ab.2013.02.024

Analyses for diagnosis and monitoring of pathological conditions often rely on blood samples, partly due to relative ease of collection. However, many interfering substances largely preclude the use of whole blood itself, necessitating separation of... Read More about Use of whole blood for analysis of disease-associated biomarkers.

Multiple myeloma: The bone marrow microenvironment and its relation to treatment (2013)
Journal Article
Andrews, S. W., Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2013). Multiple myeloma: The bone marrow microenvironment and its relation to treatment. British Journal of Biomedical Science, 70(3), 110-120. https://doi.org/10.1080/09674845.2013.11669945

Multiple myeloma is the most common haematological malignancy yet currently it remains incurable. For decades the mainstay in therapy has been non-targeted approaches including genotoxic agents and immunosuppressants. With myeloma predominantly affec... Read More about Multiple myeloma: The bone marrow microenvironment and its relation to treatment.

Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells (2012)
Journal Article
May, J. E., Morse, H. R., Xu, J., & Donaldson, C. (2012). Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells. Toxicology and Applied Pharmacology, 263(3), 374-389. https://doi.org/10.1016/j.taap.2012.07.013

There is an increasing need for development of physiologically relevant in-vitro models for testing toxicity, however determining toxic effects of agents which undergo extensive hepatic metabolism can be particularly challenging. If a source of such... Read More about Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells.

Chemotherapy-induced damage to mesenchymal stem cells: An in vitro model (2011)
Presentation / Conference
May, J. E., Morse, R., Xu, J., Avent, N. D., Febrey, S., Cox, C., …Donaldson, C. (2011, November). Chemotherapy-induced damage to mesenchymal stem cells: An in vitro model. Poster presented at In Vitro Toxicology Society Annual Meeting, Liverpool, UK

An in vitro model of chemotherapeutic damage to mesenchymal stem cells (2010)
Presentation / Conference
May, J. E., Xu, J., Morse, R., Avent, N. D., Cox, C., Wexler, S. A., & Donaldson, C. (2010, April). An in vitro model of chemotherapeutic damage to mesenchymal stem cells. Poster presented at 4th UK Mesenchymal Stem Cell Meeting, Leeds University, UK

Chemotherapeutic damage to mesenchymal stem cells (2010)
Presentation / Conference
May, J. E., & Donaldson, C. (2010, March). Chemotherapeutic damage to mesenchymal stem cells. Poster presented at SET for Britain Event 2010, House of Commons, London, UK

Mesenchymal stem cell response to chemotherapeutic damage (2009)
Presentation / Conference
May, J. E., Xu, J., Morse, R., Avent, N. D., Cox, C., Wexler, S. A., & Donaldson, C. (2009, March). Mesenchymal stem cell response to chemotherapeutic damage. Paper presented at 35th Annual meeting EBMT and 25th Meeting of the Nurses Group, Goteborg, Sweden

Toxicity testing: The search for an in vitro alternative to animal testing (2009)
Journal Article
May, J. E., Xu, J., Morse, R., Avent, N. D., & Donaldson, C. (2009). Toxicity testing: The search for an in vitro alternative to animal testing. British Journal of Biomedical Science, 66(3), 160-165. https://doi.org/10.1080/09674845.2009.11730265

Prior to introduction to the clinic, pharmaceuticals must undergo rigorous toxicity testing to ensure their safety. Traditionally, this has been achieved using in vivo animal models. However, besides ethical reasons, there is a continual drive to red... Read More about Toxicity testing: The search for an in vitro alternative to animal testing.