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Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia

Steven, Joshua; Taylor, Amy; Dean, Sarah; May, Jennifer; Anderson, Elizabeth

Authors

Joshua Steven

Amy Taylor

Sarah Dean Sarah4.Dean@uwe.ac.uk
Senior Lecturer in Biomedical Science

Jennifer May Jennifer2.May@uwe.ac.uk
Senior Lecturer in Biomedical Science



Abstract

Treatment success in leukaemia is impacted by patient resistance to chemotherapy including cytarabine (ara-C) and the anthracycline daunorubicin (DNR) requiring rapid pre-screening testing. DNR is converted to daunorubicinol (DNRol) by carbonyl reductase (CR1) in resistant patients leading to cardiomyopathy. A major mechanism for DNR resistance is p-glycoprotein (p-gp) over-expression, however the exact substrate for p-gp is unknown. The aim of this study was to characterise a novel anthracycline-resistant (K562R) cell line for mode of resistance and assess intracellular drug kinetics in a novel combined fluorescence/bioluminescence assay. IC50 values for DNR treatment were significantly higher for K562R (8.4 μM) compared to wildtype (4.8 μM) (p

Journal Article Type Meeting Abstract
Publication Date Nov 1, 2017
Print ISSN 0267-8357
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 32
Issue 6
Pages 627
APA6 Citation May, J., Steven, J., Taylor, A., Dean, S., & Anderson, E. (2017). Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia. Mutagenesis, 32(6), 627. https://doi.org/10.1093/mutage/gex033
DOI https://doi.org/10.1093/mutage/gex033
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