All Outputs (34)

A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana (2002)
Journal Article

The plant hormone abscisic acid (ABA), synthesized in response to water-deficit stress, induces stomatal closure via activation of complex signaling cascades. Recent work has established that nitric oxide (NO) is a key signaling molecule mediating AB... Read More about A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana.

Highly sensitive mixed oxide sensors for the detection of ethanol (2002)
Journal Article

Sensors consisting of mixtures of tin dioxide and zinc oxide powders in a range of proportions were constructed. Each mixture was applied to an electrode-bearing alumina substrate either as a paste, or by screen printing. The responses of these senso... Read More about Highly sensitive mixed oxide sensors for the detection of ethanol.

Multifunctional zinc finger proteins in development and disease (2002)
Journal Article

Post-transcriptional processes contribute significantly towards the generation of proteomic diversity. An increasing number of mutations have been described that affect genes encoding components of the post-transcriptional machinery. In particular, m... Read More about Multifunctional zinc finger proteins in development and disease.

Experimental logical gates in a reaction-diffusion medium: The XOR gate and beyond (2002)
Journal Article

We exploit the particulars of diffusive wave front interactions in certain types of two-reactant reaction-diffusion medium to construct a laboratory prototype of an XOR gate. In the design, the values of the logic variables are represented by the pre... Read More about Experimental logical gates in a reaction-diffusion medium: The XOR gate and beyond.

Collision-free path planning in the Belousov-Zhabotinsky medium assisted by a cellular automaton (2002)
Journal Article

We offer a new approach to computing a shortest collision-free path in a space containing obstacles, using an experimental chemical processor, based on the Belousov-Zhabotinsky (BZ) reaction. The chemical processor was then coupled via optical links... Read More about Collision-free path planning in the Belousov-Zhabotinsky medium assisted by a cellular automaton.

Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms (2002)
Journal Article

The three-dimensional structures of the isoleucine ketimine and the pyridoxamine phosphate forms of human mitochondrial branched chain aminotransferase (hBCATm) have been determined crystallographically at 1.9 Å resolution. The hBCATm-catalyzed trans... Read More about Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: Ketimine and pyridoxamine phosphate forms.

Antimicrobial properties of milk: Dependence on presence of xanthine oxidase and nitrite (2002)
Journal Article

Human and bovine milk inhibited the metabolic activity of Escherichia coli, as shown by luminescence monitoring of constructs expressing the luxCDABE genes. Inhibition was dependent on both xanthine oxidase (XO) activity and on the presence of nitrit... Read More about Antimicrobial properties of milk: Dependence on presence of xanthine oxidase and nitrite.

Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases (2002)
Journal Article

The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass are notably different when mobile-phase p... Read More about Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.

A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases (2002)
Journal Article

A new, continuous 96-well plate spectrophotometric assay for the branched-chain amino acid aminotransferases is described. Transamination of L-leucine with α-ketoglutarate results in formation of α-ketoisocaproate, which is reductively aminated back... Read More about A continuous 96-well plate spectrophotometric assay for branched-chain amino acid aminotransferases.

Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors: A comparative study involving results from laboratories in 21 countries (2002)
Journal Article

Variation in assay sensitivity was studied in more than 90 laboratories that assayed 4formalin-fixed, paraffin-processed breast and ovarian carcinoma cell lines with graded levels of HER-2/neu protein overexpression and known levels of HER-2/neu gene... Read More about Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors: A comparative study involving results from laboratories in 21 countries.

The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy (2002)
Journal Article

The chemical and biochemical oxygen demand values of a number of synthetic and wastewater samples were determined using fluorescence spectroscopy. Treated and untreated sewage samples were obtained from a local sewage treatment works while synthetic... Read More about The differentiation of biodegradable and non-biodegradable dissolved organic matter in wastewaters using fluorescence spectroscopy.

Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase (2002)
Journal Article

The human mitochondrial branched chain aminotransferase isoenzyme (hBCATm) must be stored in a reducing environment to remain active. Oxidation or labeling of hBCATm with sulfhydryl reagents results in enzyme inhibition. In this study, we investigate... Read More about Identification of a peroxide-sensitive redox switch at the CXXC motif in the human mitochondrial branched chain aminotransferase.

Phytoextractio of 137Cs: The effect of soil 137Cs concentration on 137Cs uptake by Beta vulgaris (2002)
Journal Article

The last phase for MAGNOX ELECTRIC plc's reactor decommissioning strategy is site clearance and delicencing. Phytoextraction of 137Cs may be able to assist in meeting this objective. Field trials using Beta vulgaris conducted at Bradwell Nuclear Powe... Read More about Phytoextractio of 137Cs: The effect of soil 137Cs concentration on 137Cs uptake by Beta vulgaris.

Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode (2002)
Journal Article

Screen-printed carbon electrodes (SPCEs), without chemical modification, have been investigated as disposable sensors for the measurement of trace levels of Cu2+. Cyclic voltammetry was employed to elucidate the electrochemical behaviour of Cu2+ at t... Read More about Voltammetric behaviour and trace determination of copper at a mercury-free screen-printed carbon electrode.

Experimental reaction-diffusion pre-processor for shape recognition (2002)
Journal Article

We have produced an experimental implementation of a massively-parallel reaction-diffusion processor which performs one of the most essential parts of shape recognition - construction of a skeleton. A skeleton is a unique, stable and invariant repres... Read More about Experimental reaction-diffusion pre-processor for shape recognition.

Hydrogen peroxide and nitric oxide as signalling molecules in plants (2002)
Journal Article

It is now clear that hydrogen peroxide (H2O2) and nitric oxide (NO) function as signalling molecules in plants. A wide range of abiotic and biotic stresses results in H2O2 generation, from a variety of sources. H2O2 is removed from cells via a number... Read More about Hydrogen peroxide and nitric oxide as signalling molecules in plants.

The synthesis of 3-octadecyl- and 3-docosylpyrrole, their polymerisation and incorporation into novel composite gas sensitive resistors (2002)
Journal Article

3-Octadecylpyrrole and the novel 3-docosylpyrrole were synthesised in high yields. These monomers were polymerised with various oxidising agents to produce a range of 3-octadecylpolypyrroles and 3-docosylpolypyrroles with different counter-ions. Thes... Read More about The synthesis of 3-octadecyl- and 3-docosylpyrrole, their polymerisation and incorporation into novel composite gas sensitive resistors.

Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes (2002)
Journal Article

Skatole, a derivative of tryptophan, is produced in the hind-gut of pigs and is metabolised via hepatic cytochrome P4502E1 (CYP2E1). Excessive accumulation of skatole together with androstenone, a metabolite of testosterone, in adipose tissue in some... Read More about Cytochrome P450IIE1 (CYP2E1) is induced by skatole and this induction is blocked by androstenone in isolated pig hepatocytes.

The fabrication and characterisation of a highly sensitive polypyrrole sensor and its electrical responses to amines of differing basicity at high humidities (2002)
Journal Article

Ammonia is a common feedstock for many chemical processes involving nitrogen compounds. It is a poisonous, colourless gas with a pungent smell and a fairly low odour threshold of approximately 5 ppm. The recommended threshold limit value for human ex... Read More about The fabrication and characterisation of a highly sensitive polypyrrole sensor and its electrical responses to amines of differing basicity at high humidities.

A formalin-fixed, paraffin-processed cell line standard for quality control of immunohistochemical assay of HER-2/neu expression in breast cancer (2002)
Journal Article

To ensure the accuracy and reproducibility of immunohistochemical assays for determining HER-2/neu status of patients with breast cancer, a reliable standard for monitoring assay sensitivity is necessary. We optimally fixed and paraffin processed hum... Read More about A formalin-fixed, paraffin-processed cell line standard for quality control of immunohistochemical assay of HER-2/neu expression in breast cancer.