Fabrication of microband glucose biosensors using a screen-printing water-based carbon ink and their application in serum analysis

Pemberton, Roy; Pittson, R.; Hart, John P.; Biddle, N.

Abstract

Microband glucose biosensors were fabricated by screen-printing a water-based carbon ink formulation containing cobalt phthalocyanine redox mediator and glucose oxidase (GOD) enzyme, then insulating and sectioning through the thick (20 μm) film to expose a 3 mm-long working electrode edge. The performance of these biosensors for glucose analysis was investigated at 25 °C. Voltammetry in glucose-containing buffer solutions established that an operating potential of +0.4 V vs. Ag/AgCl was suitable for analysis under both stirring and quiescent conditions. The influence of pH on biosensor performance was established and an operational pH of 8.0 was selected. Steady-state responses were obtained under quiescent conditions, suggesting a mixed mechanism predominated by radial diffusion, indicative of microelectrode behaviour. Calibration studies obtained with these biosensors showed steady-state currents that were linearly dependent on glucose concentration from the limit of detection (0.27 mM) up to 2.0 mM, with a precision for replicate biosensors of 6.2-10.7%. When applied to the determination of glucose in human serum, the concentration compared favourably to that determined by a spectroscopic method. These results have demonstrated a simple means of fabricating biosensors for glucose measurement and determination in situations where low-current real-time monitoring under quiescent conditions would be desirable. © 2008 Elsevier B.V. All rights reserved.

• Journal Article Type: Article
• Publication Date: Jan 1, 2009
• Journal: Biosensors and Bioelectronics
• Print ISSN: 0956-5663
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 24
• Issue: 5
• Pages: 1246-1252
• DOI: https://doi.org/10.1016/j.bios.2008.07.035
• Keywords: glucose biosensor, screen-printing, water-based carbon ink, microband electrode
• Public URL: https://uwe-repository.worktribe.com/output/999345
• Publisher URL: http://dx.doi.org/10.1016/j.bios.2008.07.035