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Characterization of the Salmonella bacteriophage vB_SenS-Ent1

Turner, Dann; Hezwani, M; Nelson, Shona; Salisbury, Vyv; Reynolds, Darren M.

Authors

Dann Turner Dann2.Turner@uwe.ac.uk
Senior Lecturer in Molecular Biology

M Hezwani mohammed2.hezwani@uwe.ac.uk

Vyv Salisbury vyv.salisbury@uwe.ac.uk



Abstract

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10−9 ml min−1. Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3.

Citation

Turner, D., Hezwani, M., Nelson, S., Salisbury, V., & Reynolds, D. M. (2012). Characterization of the Salmonella bacteriophage vB_SenS-Ent1. Journal of General Virology, 93(9), 2046-2056. https://doi.org/10.1099/vir.0.043331-0

Journal Article Type Article
Publication Date Sep 1, 2012
Journal Journal of General Virology
Print ISSN 0022-1317
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 93
Issue 9
Pages 2046-2056
DOI https://doi.org/10.1099/vir.0.043331-0
Keywords Salmonella, bacteriophage, vB_SenS-Ent1
Public URL https://uwe-repository.worktribe.com/output/944653
Publisher URL http://dx.doi.org/10.1099/vir.0.043331-0