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661W photoreceptor cell line as a cell model for studying retinal ciliopathies

Wheway, Gabrielle; Nazlamova, Liliya; Turner, Dann; Cross, Stephen

Authors

Gabrielle Wheway

Liliya Nazlamova

Dann Turner Dann2.Turner@uwe.ac.uk
Senior Lecturer in Molecular Biology

Stephen Cross



Abstract

Copyright © 2019 Wheway, Nazlamova, Turner and Cross. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. The retina contains several ciliated cell types, including the retinal pigment epithelium (RPE) and photoreceptor cells. The photoreceptor cilium is one of the most highly modified sensory cilia in the human body. The outer segment of the photoreceptor is a highly elaborate primary cilium, containing stacks or folds of membrane where the photopigment molecules are located. Perhaps unsurprisingly, defects in cilia often lead to retinal phenotypes, either as part of syndromic conditions involving other organs, or in isolation in the so-called retinal ciliopathies. The study of retinal ciliopathies has been limited by a lack of retinal cell lines. RPE1 retinal pigment epithelial cell line is commonly used in such studies, but the existence of a photoreceptor cell line has largely been neglected in the retinal ciliopathy field. 661W cone photoreceptor cells, derived from mouse, have been widely used as a model for studying macular degeneration, but not described as a model for studying retinal ciliopathies such as retinitis pigmentosa. Here, we characterize the 661W cell line as a model for studying retinal ciliopathies. We fully characterize the expression profile of these cells, using whole transcriptome RNA sequencing, and provide this data on Gene Expression Omnibus for the advantage of the scientific community. We show that these cells express the majority of markers of cone cell origin. Using immunostaining and confocal microscopy, alongside scanning electron microscopy, we show that these cells grow long primary cilia, reminiscent of photoreceptor outer segments, and localize many cilium proteins to the axoneme, membrane and transition zone. We show that siRNA knockdown of cilia genes Ift88 results in loss of cilia, and that this can be assayed by high-throughput screening. We present evidence that the 661W cell line is a useful cell model for studying retinal ciliopathies.

Citation

Wheway, G., Nazlamova, L., Turner, D., & Cross, S. (in press). 661W photoreceptor cell line as a cell model for studying retinal ciliopathies. Frontiers in Genetics, 10(APR), https://doi.org/10.3389/fgene.2019.00308

Journal Article Type Article
Acceptance Date Mar 21, 2019
Online Publication Date Apr 5, 2019
Publicly Available Date Nov 30, -0001
Journal Frontiers in Genetics
Electronic ISSN 1664-8021
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 10
Issue APR
DOI https://doi.org/10.3389/fgene.2019.00308
Keywords retina, photoreceptor, cilia, ciliopathy, cell model, retinitis pigmentosa
Public URL https://uwe-repository.worktribe.com/output/849042
Publisher URL https://doi.org/10.3389/fgene.2019.00308

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