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Pectobacterium atrosepticum phage vB_PatP_CB5: A member of the proposed genus ‘Phimunavirus’

Buttimer, Colin; Lucid, Alan; Neve, Horst; Franz, Charles; O’Mahony, Jim; Turner, Dann; Lavigne, Rob; Coffey, Aidan

Authors

Colin Buttimer

Alan Lucid

Horst Neve

Charles Franz

Jim O’Mahony

Dann Turner Dann2.Turner@uwe.ac.uk
Senior Lecturer in Molecular Biology

Rob Lavigne

Aidan Coffey



Abstract

© 2018 by the authors. Licensee MDPI, Basel, Switzerland. Pectobacterium atrosepticum is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the Pectobacterium phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indicate that it belongs to the Podoviridae family. CB5 shares significant pairwise nucleotide identity (≥80%) with P. atrosepticum phages 'M1, Peat1, and PP90 and also shares common genome organization. Phylograms constructed using conserved proteins and whole-genome comparison-based amino acid sequences show that these phages form a distinct clade within the Autographivirinae. They also possess conserved RNA polymerase recognition and specificity loop sequences. Their lysis cassette resembles that of KP34virus, containing in sequential order a U-spanin, a holin, and a signal–arrest–release (SAR) endolysin. However, they share low pairwise nucleotide identity with the type phage of the KP34virus genus, Klebsiella phage KP34. In addition, phage KP34 does not possess several conserved proteins associated with these P. atrosepticum phages. As such, we propose the allocation of phages CB5, Peat1, 'M1, and PP90 to a separate new genus designated Phimunavirus.

Citation

Buttimer, C., Lucid, A., Neve, H., Franz, C., O’Mahony, J., Turner, D., …Coffey, A. (2018). Pectobacterium atrosepticum phage vB_PatP_CB5: A member of the proposed genus ‘Phimunavirus’. Viruses, 10(8), 394. https://doi.org/10.3390/v10080394

Journal Article Type Article
Acceptance Date Jul 24, 2018
Publication Date Aug 1, 2018
Publicly Available Date Nov 30, -0001
Journal Viruses
Electronic ISSN 1999-4915
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 10
Issue 8
Pages 394
DOI https://doi.org/10.3390/v10080394
Keywords Pectobacterium atrosepticum, Autographivirinae, ‘Phimunavirus’, bacteriophage, phage, Podoviridae, taxonomy
Public URL https://uwe-repository.worktribe.com/output/864113
Publisher URL http://dx.doi.org/10.3390/v10080394

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