A novel bioluminescent bacterial biosensor for measurement of Ara-CTP and cytarabine potentiation by fludarabine in seven leukaemic cell lines

Conway, Myra; Anderson, Elizabeth; Smith, M. Ann; Martin, Ashley; Ruddock, Mark; Lamont, John; Alloush, Habib; Conway, Myra E.; Mehta, Priyanka; Smith, J. Graham; Salisbury, Vyv

Abstract

This study evaluates an in vitro biosensor assay capable of detecting the intracellular levels of the tri-phosphorylated form of cytarabine (Ara-CTP) within one working day. The biosensor predicted the response of seven leukaemic cell lines with varying known sensitivities to cytarabine alone and in combination with fludarabine. High-performance liquid chromatography (HPLC), 3-day assessment of cellular viable mass, and flow cytometric assessment of apoptosis were used to validate biosensor performance. A correlation between the biosensor results and Ara-CTP quantitation by HPLC was confirmed (R= 0.972). The biosensor was also capable of detecting enhanced accumulation of Ara-CTP following sequential pre-treatment of leukaemic cells with cytarabine ± fludarabine. © 2013 Elsevier Ltd.

• Journal Article Type: Article
• Publication Date: Jun 1, 2013
• Journal: Leukemia Research
• Print ISSN: 0145-2126
• Electronic ISSN: 1873-5835
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 37
• Issue: 6
• Pages: 690-696
• DOI: https://doi.org/10.1016/j.leukres.2013.02.012
• Keywords: acute myeloid leukaemia, biosensor, ara-CTP
• Public URL: https://uwe-repository.worktribe.com/output/931278
• Publisher URL: http://dx.doi.org/10.1016/j.leukres.2013.02.012