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In planta induced changes in the native plasmid profile of Pseudomonas syringae pathover phaseolicola strain 1302A

Slater, Ross T.; Neale, Helen C.; Neale, Helen; Slater, Ross; Manoharan, Bharani; Mayne, Lisa Marie; Arnold, Dawn L.

Authors

Ross T. Slater

Helen C. Neale

Helen Neale Helen2.Neale@uwe.ac.uk
Occasional Associate Lecturer - HAS DAS

Ross Slater

Bharani Manoharan

Lisa Marie Mayne

Dawn Arnold Dawn.Arnold@uwe.ac.uk
Professor in Molecular Plant Pathology



Abstract

Pseudomonas syringae pv. phaseolicola (Pph) strain 1302A, a causative agent of halo blight in the common bean Phaseolus vulgaris, contains four native plasmids designated pAV505 (150. kb), pAV506 (50. kb), pAV507 (47. kb) and pAV508 (42. kb). Pph 1302A also contains a 106. kb genomic island PPHGI-1 which shares features with integrative and conjugative elements (ICElands) and carries the effector gene avrPphB (hopAR1) which triggers a defensive response in bean cultivars carrying the matching R3 resistance gene. It has been shown that when Pph 1302A is sequentially inoculated (passaged) through resistant bean cultivar Tendergreen (TG) in which the hypersensitive response (HR) is generated, the three largest plasmids are lost and an extra ~100. kb plasmid is gained, which tests confirmed to be the 106. kb circular form of PPHGI-1. The aim of the current study was to determine if upon further passaging though bean plants, the plasmid profile of Pph 1302A would alter again and if the missing plasmids had been integrated into the chromosome. Pph 1302A-P6, the strain with the altered plasmid profile was passaged twice through TG and of the four re-isolated strains examined all displayed the plasmid profile associated with wildtype Pph 1302A, that is, all four native plasmids had reappeared and the PPHGI-1 plasmid was absent. This demonstrated that the plasmid composition of Pph 1302A-P6 could indeed change on further exposure to the plant environment and also that the seemingly missing native plasmids were still present within the genome, lending evidence to the theory that they had integrated into the chromosome. Furthermore two of these re-isolated strains had lost PPHGI-1 entirely, meaning they no longer triggered a HR on TG and instead generated a disease response. This study clearly demonstrates the plasticity of the bacterial genome and the extent it can be influenced by the plant environment and conditions generated during the HR. © 2013 Elsevier Inc.

Citation

Slater, R. T., Neale, H. C., Neale, H., Slater, R., Mayne, L. M., Manoharan, B., & Arnold, D. L. (2013). In planta induced changes in the native plasmid profile of Pseudomonas syringae pathover phaseolicola strain 1302A. Plasmid, 70(3), 420-424. https://doi.org/10.1016/j.plasmid.2013.07.002

Journal Article Type Article
Publication Date Nov 1, 2013
Journal Plasmid
Print ISSN 0147-619X
Electronic ISSN 1095-9890
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 70
Issue 3
Pages 420-424
DOI https://doi.org/10.1016/j.plasmid.2013.07.002
Keywords native plasmids, plasmid profile, genomic island, pseudomonas syringae pv. phaseolicola
Public URL https://uwe-repository.worktribe.com/output/926353
Publisher URL http://dx.doi.org/10.1016/j.plasmid.2013.07.002