Scoping studies to establish the capability and utility of a real-time bioaerosol sensor to characterise emissions from environmental sources

Nasir, Zaheer Ahmad; Hayes, Enda; Williams, Ben; Gladding, Toni; Rolph, Catherine; Khera, Shagun; Jackson, Simon; Bennett, Allan; Collins, Samuel; Parks, Simon; Attwood, Alexis; Kinnersley, Robert; Walsh, Kerry; Garcia-Alcega, Sonia; Pollard, Simon; Drew, Gill; Coulon, Frederic; Tyrrel, Sean

Abstract

A novel dual excitation wavelength based bioaerosol sensor with multiple fluorescence bands called Spectral Intensity Bioaerosol Sensor (SIBS) has been assessed across five contrasting outdoor environments. The mean concentrations of total and fluorescent particles across the sites were highly variable being the highest at the agricultural farm (2.6 cm−3 and 0.48 cm−3, respectively) and the composting site (2.32 cm−3 and 0.46 cm−3, respectively) and the lowest at the dairy farm(1.03 cm−3 and 0.24 cm−3, respectively) and the sewage treatment works (1.03 cm−3 and 0.25 cm−3, respectively). In contrast, thenumber-weighted fluorescent fraction was lowest at the agricultural site (0.18) in comparison to the other sites indicating high variability in nature and magnitude of emissions from environmental sources. The fluorescence emissions data demonstrated that the spectra at different sites were multimodal with intensity differences largely at wavelengths located in secondary emission peaks for λex 280 and λex 370. This finding suggests differences in the molecular composition of emissions at these sites which can help to identify distinct fluorescence signature of different environmental sources. Overall this study demonstrated that SIBS provides additional spectral information compared to existing instruments and capability to resolve spectrally integrated signals fromrelevant biological fluorophores could improve selectivity and thus enhance discrimination and classification strategies for real-time characterisation of bioaerosols

Citation

Nasir, Z. A., Hayes, E., Williams, B., Gladding, T., Rolph, C., Khera, S., …Tyrrel, S. (2019). Scoping studies to establish the capability and utility of a real-time bioaerosol sensor to characterise emissions from environmental sources. Science of the Total Environment, 648, 25-32

• Journal Article Type: Article
• Acceptance Date: Jul 30, 2018
• Online Publication Date: Aug 9, 2018
• Publication Date: Jan 15, 2019
• Deposit Date: Aug 29, 2018
• Publicly Available Date: Aug 29, 2018
• Journal: Science of the Total Environment
• Print ISSN: 0048-9697
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 648
• Pages: 25-32
• Keywords: real-time monitoring, bioaerosols, emissions characterisation, fluorescence spectra
• Public URL: https://uwe-repository.worktribe.com/output/853903