Skip to main content

Research Repository

Advanced Search

Sniffing out resistance – Rapid identification of urinary tract infection-causing bacteria and their antibiotic susceptibility using volatile metabolite profiles

Smart, Amy; de Lacy Costello, Ben; White, Paul; Avison, Matthew; Batty, Claire; Turner, Claire; Persad, Raj; Ratcliffe, Norman

Sniffing out resistance – Rapid identification of urinary tract infection-causing bacteria and their antibiotic susceptibility using volatile metabolite profiles Thumbnail


Authors

Amy Smart

Paul White Paul.White@uwe.ac.uk
Professor in Applied Statistics

Matthew Avison

Claire Batty

Claire Turner

Raj Persad

Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science



Abstract

© 2019 Elsevier B.V. Antibiotic resistance is set to be an unprecedented threat to modern medicine. ‘Sniffing’ bacteria potentially offers a rapid way to determine susceptibility. A successful proof-of-principle study is described, using thermal desorption-gas chromatography-mass spectrometry (TD–GC–MS) to ‘smell’ cephalexin and ciprofloxacin resistant and sensitive Urinary Tract Infection (UTI)-causing bacteria. 578 peaks at unique retention times were detected from 86 chromatograms of 18 bacterial isolates (E. coli, K. pneumoniae and P. aeruginosa). The isolates were grown with and without the presence of antibiotic. Chi-square analysis found 9 compounds that differed significantly between cephalexin sensitive and resistant isolates, and 22 compounds that differed significantly between ciprofloxacin sensitive and resistant isolates, at p ≤ 0.05. When antibiotic was added to the media, more differences were found in the cephalexin group, attributed to lysis, but not in the ciprofloxacin group. Further work with large sample sizes will potentially enable the development of diagnostic algorithms using presence/absence of particular compounds of interest.

Citation

Smart, A., de Lacy Costello, B., White, P., Avison, M., Batty, C., Turner, C., …Ratcliffe, N. (2019). Sniffing out resistance – Rapid identification of urinary tract infection-causing bacteria and their antibiotic susceptibility using volatile metabolite profiles. Journal of Pharmaceutical and Biomedical Analysis, 167, 59-65. https://doi.org/10.1016/j.jpba.2019.01.044

Journal Article Type Article
Acceptance Date Jan 26, 2019
Online Publication Date Jan 31, 2019
Publication Date Apr 15, 2019
Deposit Date Feb 14, 2019
Publicly Available Date Mar 29, 2024
Journal Journal of Pharmaceutical and Biomedical Analysis
Print ISSN 0731-7085
Electronic ISSN 1873-264X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 167
Pages 59-65
DOI https://doi.org/10.1016/j.jpba.2019.01.044
Keywords volatile, metabolite, profiles, smell, thermal desorption, gas chromatography, mass spectrometry, antibiotic, resistance, urinary tract infection, susceptibility, cephalexin, ciprofloxacin
Public URL https://uwe-repository.worktribe.com/output/848837
Publisher URL https://doi.org/10.1016/j.jpba.2019.01.044

Files







You might also like



Downloadable Citations