Classification of the mechanism of toxicity as applied to human cell line ECV304

Djawad, Yasser Abd; Kiely, Janice; Luxton, Richard

Abstract

© 2020 Informa UK Limited, trading as Taylor & Francis Group. The objective of this study was to identify the pattern of cytotoxicity testing of the human cell line ECV304 using three techniques of an ensemble learning algorithm (bagging, boosting and stacking). The study of cell morphology of ECV304 cell line was conducted using impedimetric measurement. Three types of toxins were applied to the ECV304 cell line namely 1 mM hydrogen peroxide (H2O2), 5% dimethyl sulfoxide and 10 μg Saponin. The measurement was conducted using electrodes and lock-in amplifier to detect impedance changes during cytotoxicity testing within a frequency range 200 and 830 kHz. The results were analysed, processed and extracted using detrended fluctuation analysis to obtain characteristics and features of the cells when exposed to the each of the toxins. Three ensemble algorithms applied showed slightly different results on the performance for classifying the data set from the feature extraction that was performed. However, the results show that the cell reaction to the toxins could be classified.

Citation

Djawad, Y. A., Kiely, J., & Luxton, R. (2021). Classification of the mechanism of toxicity as applied to human cell line ECV304. Computer Methods in Biomechanics and Biomedical Engineering, 24(9), 933-944. https://doi.org/10.1080/10255842.2020.1861255

• Journal Article Type: Article
• Acceptance Date: Dec 3, 2020
• Online Publication Date: Dec 27, 2020
• Publication Date: 2021
• Deposit Date: Jan 14, 2021
• Publicly Available Date: Dec 28, 2021
• Journal: Computer Methods in Biomechanics and Biomedical Engineering
• Print ISSN: 1025-5842
• Electronic ISSN: 1476-8259
• Publisher: Taylor & Francis
• Peer Reviewed: Peer Reviewed
• Volume: 24
• Issue: 9
• Pages: 933-944
• DOI: https://doi.org/10.1080/10255842.2020.1861255
• Keywords: Human-Computer Interaction; Bioengineering; Biomedical Engineering; General Medicine; Computer Science Applications
• Public URL: https://uwe-repository.worktribe.com/output/6967956
• Additional Information: Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=gcmb20; Received: 2020-04-13; Revised: 2020-10-28; Accepted: 2020-12-03; Published: 2020-12-27