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Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration

Targett, Imogen L; Crompton, Lucy A; Conway, Myra E; Craig, Tim J

Authors

Imogen L Targett

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Dr Lucy Crompton Lucy.Crompton@uwe.ac.uk
Senior Lecturer in Biomedical Sciences (Neuroscience)

Myra Conway Myra.Conway@uwe.ac.uk
Occasional Associate Lecturer - CHSS - DAS

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Dr Tim Craig Tim.Craig@uwe.ac.uk
Associate Professor of Neuroscience



Abstract

There has been much interest in the use of cell culture models of neurones, to avoid the animal welfare and cost issues of using primary and human-induced pluripotent stem cell (hiPSC)-derived neurones respectively. The human neuroblastoma cell line, SH-SY5Y, is extensively used in laboratories as they can be readily expanded, are of low cost and can be differentiated into neurone-like cells. However, much debate remains as to their phenotype once differentiated, and their ability to recapitulate the physiology of bona fide neurones. Here, we characterise a differentiation protocol using retinoic acid and BDNF, which results in extensive neurite outgrowth/branching within 10 days, and expression of key neuronal and synaptic markers. We propose that these differentiated SH-SY5Y cells may be a useful substitute for primary or hiPSC-derived neurones for cell biology studies, in order to reduce costs and animal usage. We further propose that this characterised differentiation timecourse could be used as an in vitro model for neuronal differentiation, for proof-of principle studies on neurogenesis, e.g. relating to neurodegenerative diseases. Finally, we demonstrate profound changes in Tau phosphorylation during differentiation of these cells, suggesting that they should not be used for neurodegeneration studies in their undifferentiated state.

Citation

Targett, I. L., Crompton, L. A., Conway, M. E., & Craig, T. J. (in press). Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration. In Vitro Cellular and Developmental Biology - Animal,

Journal Article Type Article
Acceptance Date Jun 20, 2024
Deposit Date Jun 21, 2024
Journal In Vitro Cellular & Developmental Biology - Animal
Print ISSN 1071-2690
Publisher Society for In-Vitro Biology
Peer Reviewed Peer Reviewed
Keywords SH-SY5Y; neuronal differentiation; cholinergic; synaptic markers; neurodegeneration
Public URL https://uwe-repository.worktribe.com/output/12079454
Additional Information Paper accepted but not yet published, so no DOI