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In vitro and in vivo evaluation of the biofilm-degrading Pseudomonas phage Motto, as a candidate for phage therapy

Manohar, Prasanth; Loh, Belinda; Turner, Dann; Tamizhselvi, Ramasamy; Mathankumar, Marimuthu; Elangovan, Namasivayam; Nachimuthu, Ramesh; Leptihn, Sebastian

In vitro and in vivo evaluation of the biofilm-degrading Pseudomonas phage Motto, as a candidate for phage therapy Thumbnail


Authors

Prasanth Manohar

Belinda Loh

Ramasamy Tamizhselvi

Marimuthu Mathankumar

Namasivayam Elangovan

Ramesh Nachimuthu

Sebastian Leptihn



Abstract

Infections caused by Pseudomonas aeruginosa are becoming increasingly difficult to treat due to the emergence of strains that have acquired multidrug resistance. Therefore, phage therapy has gained attention as an alternative to the treatment of pseudomonal infections. Phages are not only bactericidal but occasionally show activity against biofilm as well. In this study, we describe the Pseudomonas phage Motto, a T1-like phage that can clear P. aeruginosa infections in an animal model and also exhibits biofilm-degrading properties. The phage has a substantial anti-biofilm activity against strong biofilm-producing isolates (n = 10), with at least a twofold reduction within 24 h. To demonstrate the safety of using phage Motto, cytotoxicity studies were conducted with human cell lines (HEK 293 and RAW 264.7 macrophages). Using a previously established in vivo model, we demonstrated the efficacy of Motto in Caenorhabditis elegans, with a 90% survival rate when treated with the phage at a multiplicity of infection of 10.

Citation

Manohar, P., Loh, B., Turner, D., Tamizhselvi, R., Mathankumar, M., Elangovan, N., …Leptihn, S. (2024). In vitro and in vivo evaluation of the biofilm-degrading Pseudomonas phage Motto, as a candidate for phage therapy. Frontiers in Microbiology, 15, Article 1344962. https://doi.org/10.3389/fmicb.2024.1344962

Journal Article Type Article
Acceptance Date Feb 22, 2024
Online Publication Date Mar 15, 2024
Publication Date Mar 15, 2024
Deposit Date Mar 19, 2024
Publicly Available Date Mar 20, 2024
Journal Frontiers in Microbiology
Print ISSN 1664-302X
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 15
Article Number 1344962
DOI https://doi.org/10.3389/fmicb.2024.1344962
Keywords Caenorhabditis elegans, bacteriophages, anti-biofilm activity, phage-mammalian cell interaction, phage therapy, Pseudomonas aeruginosa
Public URL https://uwe-repository.worktribe.com/output/11832138
Publisher URL https://www.frontiersin.org/

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