Studies towards the development of a screen-printed carbon electrochemical immunosensor array for mycotoxins: A sensor for aflatoxin B1

Pemberton, Roy; Pittson, R.; Biddle, N.; Hart, John P.; Drago, G. A.

Abstract

Screen-printed carbon electrodes (SPCEs) bearing a surface-adsorbed antibody against aflatoxin B1 (AFB1) were used in a competitive immunoassay, based on competition of free analyte with a biotinylated aflatoxin B1 conjugate. Subsequent addition of streptavidin-alkaline phosphatase (AP) conjugate, followed by a 1-naphthyl phosphate substrate resulted in the production of the electrochemically active product, 1-naphthol; this was oxidized using linear sweep voltammetry and constituted the measurement step. Investigations were carried out to test the suitability of reagents by enzyme-linked immunoassay (ELISA) and to deduce the optimum blocking agent for the immunosensors. Using the electrochemical immunosensor, a calibration plot for AFB1 was obtained over the concentration range 0.15 to 2.5 ng/mL, giving a detection limit of around 0.15 ng/mL in buffer solution. The immunosensors were fabricated in an array configuration, suitable for use in conjunction with 96-well microtiter plates; indeed, each step of the immunoassay was performed by dipping the electrodes into microwells. These results represent the initial studies towards the development of an automated instrument for multi-analyte determinations using immunosensor arrays for mycotoxin detection and form the basis for further studies. Copyright © Taylor & Francis Group, LLC.

• Presentation Conference Type: Conference Paper (published)
• Publication Date: Jun 20, 2006
• Journal: Analytical Letters
• Print ISSN: 0003-2719
• Electronic ISSN: 1532-236X
• Publisher: Taylor & Francis
• Peer Reviewed: Not Peer Reviewed
• Volume: 39
• Issue: 8
• Pages: 1573-1586
• DOI: https://doi.org/10.1080/00032710600713289
• Keywords: aflatoxin, screen‐printed carbon electrode, immunosensor, ; voltammetry
• Public URL: https://uwe-repository.worktribe.com/output/1045027