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Evaluation of microbial viability and nucleic acid integrity after exposure to new molecular transport medium (Σ-MM™)

Elcocks, Ellena; Qadir, Sheikh; Adukwu, Emmanuel

Evaluation of microbial viability and nucleic acid integrity after exposure to new molecular transport medium (Σ-MM™) Thumbnail


Authors

Ellena Elcocks

Sheikh Qadir



Abstract

A range of organisms (bacteria and yeasts) were inoculated into Sigma MMTM. The inoculum was then aliquoted into TSB broth at 1 minute and 2 minutes to assess for turbidity indicating growth over a 10 day period. A separate aliquot was also removed for molecular assessment. No turbidity was noted at 10 days for any of the organisms tested. This study demonstrated that the Sigma MMTM molecular medium is able to rapidly inactivate bacteria and yeast whilst preserving bacterial DNA.

Report Type Technical Report
Publication Date Jul 1, 2022
Deposit Date Dec 25, 2022
Publicly Available Date Jan 3, 2023
Keywords Molecular transport; microbial viability; Nucleic acid integrity; SARS-CoV-2; SARS-CoV-2 Σ-MM™ deactivating and stabilising media
Public URL https://uwe-repository.worktribe.com/output/10274453
Publisher URL https://www.mwe.co.uk/wp-content/uploads/2022/07/Sigma-MM-Clinical-Studies.pdf

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