Dr Lucy Crompton's Outputs (15)

Reactive astrocytes generated from human iPSC are pro-inflammatory and display altered metabolism (2024)
Journal Article

Astrocytes are the most abundant type of glial cell in the central nervous system and they play pivotal roles in both normal health and disease. Their dysfunction is detrimental to many brain related pathologies. Under pathological conditions, such a... Read More about Reactive astrocytes generated from human iPSC are pro-inflammatory and display altered metabolism.

Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration (2024)
Journal Article

There has been much interest in the use of cell culture models of neurones, to avoid the animal welfare and cost issues of using primary and human-induced pluripotent stem cell (hiPSC)-derived neurones respectively. The human neuroblastoma cell line,... Read More about Differentiation of SH-SY5Y neuroblastoma cells using retinoic acid and BDNF: A model for neuronal and synaptic differentiation in neurodegeneration.

Human stem cell-derived ventral midbrain astrocytes exhibit a region-specific secretory profile (2023)
Journal Article

This scientific commentary refers to ‘Human stem cell-derived astrocytes exhibit region-specific heterogeneity in their secretory profiles’, by Clarke et al. (https://doi.org/10.1093/brain/awaa258) in Brain.

Efficient and scalable generation of human ventral midbrain astrocytes from human-induced pluripotent stem cells (2021)
Journal Article

In Parkinson's disease, progressive dysfunction and degeneration of dopamine neurons in the ventral midbrain cause life-changing symptoms. Neuronal degeneration has diverse causes in Parkinson's, including non-cell autonomous mechanisms mediated by a... Read More about Efficient and scalable generation of human ventral midbrain astrocytes from human-induced pluripotent stem cells.

A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons (2020)
Journal Article

Macroautophagy/autophagy cytoplasmic quality control pathways are required during neural development and are critical for the maintenance of functional neuronal populations in the adult brain. Robust evidence now exists that declining neuronal autoph... Read More about A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons.

Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for Parkinson’s disease research (2019)
Book Chapter

To appreciate the positive or negative impact of autophagy during the initiation and progression of human diseases, the isolation or de novo generation of appropriate cell types is required to support focused in vitro assays. In human neurodegenerati... Read More about Imaging autophagy in hiPSC-derived midbrain dopaminergic neuronal cultures for Parkinson’s disease research.

Nanoparticle-induced neuronal toxicity across placental barriers is mediated by autophagy and dependent on astrocytes (2018)
Journal Article

The potential for maternal nanoparticle (NP) exposures to cause developmental toxicity in the fetus without the direct passage of NPs has previously been shown, but the mechanism remained elusive. We now demonstrate that exposure of cobalt and chromi... Read More about Nanoparticle-induced neuronal toxicity across placental barriers is mediated by autophagy and dependent on astrocytes.

Astrocytes in a dish: Using pluripotent stem cells to model neurodegenerative and neurodevelopmental disorders (2017)
Journal Article

Neuroscience and Neurobiology have historically been neuron biased, yet up to 40% of the cells in the brain are astrocytes. These cells are heterogeneous and regionally diverse but universally essential for brain homeostasis. Astrocytes regulate syna... Read More about Astrocytes in a dish: Using pluripotent stem cells to model neurodegenerative and neurodevelopmental disorders.

Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells (2014)
Journal Article

Connexin43 (Cx43) is the most widely and abundantly expressed gap junction (GJ) protein and it is strongly associated with the regulation of cell cycle progression. Emerging roles for Cx43 in cell adhesion and migration during neural differentiation... Read More about Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells.

Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling (2013)
Journal Article

Basal forebrain cholinergic neurons (bfCNs) which provide innervation to the hippocampus and cortex, are required for memory and learning, and are primarily affected in Alzheimer's Disease (AD), resulting in related cognitive decline. Therefore gener... Read More about Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling.

Signalling of DNA damage and cytokines across cell barriers exposed to nanoparticles depends on barrier thickness (2011)
Journal Article

The use of nanoparticles in medicine is ever increasing, and it is important to understand their targeted and non-targeted effects. We have previously shown that nanoparticles can cause DNA damage to cells cultured below a cellular barrier without cr... Read More about Signalling of DNA damage and cytokines across cell barriers exposed to nanoparticles depends on barrier thickness.

Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm (2011)
Journal Article

The anterior visceral endoderm (AVE), a signalling centre within the simple epithelium of the visceral endoderm (VE), is required for anterior-posterior axis specification in the mouse embryo. AVE cells migrate directionally within the VE, thereby pr... Read More about Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm.

Early embryonic expression patterns of the mouse Flamingo and Prickle orthologues (2007)
Journal Article

The Drosophila melanogaster proteins Flamingo and Prickle act in the planar cell polarity (PCP) pathway, which is required for acquisition of epithelial polarity in the wing, eye, and epidermis. In mammals, PCP signaling has been shown to regulate ce... Read More about Early embryonic expression patterns of the mouse Flamingo and Prickle orthologues.

BMP signalling inhibits premature neural differentiation in the mouse embryo (2007)
Journal Article

The specification of a subset of epiblast cells to acquire a neural fate constitutes the first step in the generation of the nervous system. Little is known about the signals required for neural induction in the mouse. We have analysed the role of BM... Read More about BMP signalling inhibits premature neural differentiation in the mouse embryo.

Global hypomethylation of the genome in XX embryonic stem cells (2005)
Journal Article

Embryonic stem (ES) cells are important tools in the study of gene function and may also become important in cell therapy applications. Establishment of stable XX ES cell lines from mouse blastocysts is relatively problematic owing to frequent loss o... Read More about Global hypomethylation of the genome in XX embryonic stem cells.