All Outputs (34)

Deletions in the MAL gene result in loss of Mal protein, defining the rare inherited AnWj-negative blood group phenotype (2024)
Journal Article

The genetic background of the high prevalence red blood cell antigen AnWj has remained unresolved since its identification in 1972, despite reported associations with both CD44 and Smyd1 histone methyltransferase. Development of anti-AnWj, which may... Read More about Deletions in the MAL gene result in loss of Mal protein, defining the rare inherited AnWj-negative blood group phenotype.

Complete absence of GLUT1 does not impair human terminal erythroid differentiation (2024)
Journal Article

The Glucose transporter 1 (GLUT1) is one of the most abundant proteins within the erythrocyte membrane and is required for glucose and dehydroascorbic acid (Vitamin C precursor) transport. It is widely recognized as a key protein for red cell structu... Read More about Complete absence of GLUT1 does not impair human terminal erythroid differentiation.

An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing (2024)
Journal Article

CRISPR-based DNA editing technologies enable rapid and accessible genome engineering of eukaryotic cells. However, the delivery of genetically encoded CRISPR components remains challenging and sustained Cas9 expression correlates with higher off-targ... Read More about An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing.

Basigin mediation of Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter 1 (2024)
Journal Article

Plasmodium falciparum invasion of the red blood cell is reliant upon the essential interaction of PfRh5 with the host receptor protein basigin. Basigin exists as part of one or more multiprotein complexes, most notably through interaction with the mo... Read More about Basigin mediation of Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter 1.

Generation of red blood cells from stem cells: Achievements, opportunities and perspectives for malaria research (2022)
Journal Article

Parasites of the genus Plasmodium that cause malaria survive within humans by invasion of, and proliferation within, the most abundant cell type in the body, the red blood cell. As obligate, intracellular parasites, interactions between parasite and... Read More about Generation of red blood cells from stem cells: Achievements, opportunities and perspectives for malaria research.

Missense mutations in PIEZO1, which encodes the Piezo1 mechanosensor protein, define Er red blood cell antigens (2022)
Journal Article

Despite the identification of the high-incidence red cell antigen Era nearly 40 years ago, the molecular background of this antigen, together with the other 2 members of the Er blood group collection, has yet to be elucidated. Whole exome and Sanger... Read More about Missense mutations in PIEZO1, which encodes the Piezo1 mechanosensor protein, define Er red blood cell antigens.

Band 3, an essential red blood cell hub of activity (2021)
Journal Article

The red blood cell (RBC) is a marvel of cellular evolutionary specialization. Whilst often considered simplistic owing to its absence of nuclei and other cellular organelles, this inability to respond transcriptionally and to replenish components thr... Read More about Band 3, an essential red blood cell hub of activity.

Rapid diagnosis of hereditary haemolytic anaemias using automated rheoscopy and supervised machine learning (2020)
Journal Article

Haemolytic anaemias arise when red blood cell (RBC) integrity is compromised, eventually resulting in premature clearance or lysis and leading to anaemia when these effects cannot be sufficiently compensated by the capacity of the bone marrow to prod... Read More about Rapid diagnosis of hereditary haemolytic anaemias using automated rheoscopy and supervised machine learning.

PIEZO1 gain-of-function mutations delay reticulocyte maturation in hereditary xerocytosis (2020)
Journal Article

Dehydrated hereditary stomatocytosis 1 or hereditary xerocytosis (HX, OMIM 194380) is a rare hereditary autosomal dominant disorder characterized by hemolytic anemia and red blood cell (RBC) dehydration. The occurrence of HX is linked with gain-of-fu... Read More about PIEZO1 gain-of-function mutations delay reticulocyte maturation in hereditary xerocytosis.

Reticulocyte and red blood cell deformation triggers specific phosphorylation events (2019)
Journal Article

The capacity to undergo substantial deformation is a defining characteristic of the red blood cell (RBC), facilitating transit through the splenic interendothelial slits and microvasculature. Establishment of this remarkable property occurs during a... Read More about Reticulocyte and red blood cell deformation triggers specific phosphorylation events.

Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements (2019)
Journal Article

Investigating the role that host erythrocyte proteins play in malaria infection is hampered by the genetic intractability of this anucleate cell. Here we report that reticulocytes derived through in vitro differentiation of an enucleation-competent i... Read More about Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements.

Non-muscle myosin ii drives vesicle loss during human reticulocyte maturation (2018)
Journal Article

The process of maturation of reticulocytes into fully mature erythrocytes that occurs in the circulation is known to be characterized by a complex interplay between loss of cell surface area and volume, removal of remnant cell organelles and redundan... Read More about Non-muscle myosin ii drives vesicle loss during human reticulocyte maturation.

Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing (2018)
Journal Article

Regular blood transfusion is the cornerstone of care for patients with red blood cell (RBC) disorders such as thalassaemia or sickle-cell disease. With repeated transfusion, alloimmunisation often occurs due to incompatibility at the level of minor b... Read More about Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing.

CD47 surface stability is sensitive to actin disruption prior to inclusion within the band 3 macrocomplex (2017)
Journal Article

CD47 is an important 'marker of self' protein with multiple isoforms produced though alternative splicing that exhibit tissue-specific expression. Mature erythrocytes express CD47 isoform 2 only, with membrane stability of this version dependent on i... Read More about CD47 surface stability is sensitive to actin disruption prior to inclusion within the band 3 macrocomplex.

Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion (2017)
Journal Article

Invasion of the red blood cell (RBC) by the Plasmodium parasite defines the start of malaria disease pathogenesis. To date, experimental investigations into invasion have focused predominantly on the role of parasite adhesins or signaling pathways an... Read More about Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells (2017)
Journal Article

With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors d... Read More about An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.

A new junctional hierarchy (2016)
Journal Article

In this issue of Blood, Lu et al report on the generation and characterization of erythrocytes from a dematin knockout mouse and demonstrate the previously unrecognized centrality of this protein to the junctional protein complex-mediated maintenance... Read More about A new junctional hierarchy.

Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts (2016)
Journal Article

Ankyrin-R provides a key link between band 3 and the spectrin cytoskeleton that helps to maintain the highly specialized erythrocyte biconcave shape. Ankyrin deficiency results in fragile spherocytic erythrocytes with reduced band 3 and protein 4.2 e... Read More about Severe Ankyrin-R deficiency results in impaired surface retention and lysosomal degradation of RhAG in human erythroblasts.

Erythrocyte invasion receptors for Plasmodium falciparum: New and old (2016)
Journal Article

Understanding the complex process by which the invasive form of the Plasmodium falciparum parasite, the merozoite, attaches to and invades erythrocytes as part of its blood stage life cycle represents a key area of research in the battle to combat ma... Read More about Erythrocyte invasion receptors for Plasmodium falciparum: New and old.

Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton (2016)
Journal Article

The invasive blood-stage malaria parasite-the merozoite-induces rapid morphological changes to the target erythrocyte during entry. However, evidence for active molecular changes in the host cell that accompany merozoite invasion is lacking. Here, we... Read More about Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton.