Claire M. Perks
Insulin receptor isoform variations in prostate cancer cells
Perks, Claire M.; Zielinska, H. A.; Wang, Jing; Jarrett, Caroline; Frankow, A.; Ladomery, Michael R.; Bahl, Amit; Rhodes, Anthony; Oxley, Jon; Holly, Jeff M. P.
Authors
H. A. Zielinska
Jing Wang
Caroline Jarrett
A. Frankow
Prof Michael Ladomery Michael.Ladomery@uwe.ac.uk
Professor of Genetics
Amit Bahl
Anthony Rhodes
Jon Oxley
Jeff M. P. Holly
Abstract
� 2016 Perks, Zielinska, Wang, Jarrett, Frankow, Ladomery, Bahl, Rhodes, Oxley and Holly. Men who develop prostate cancer (PCa) increasingly have one of the co-morbidities associated with a Western lifestyle that are characterized by hyperinsulinemia, hyperglycemia and increased expression of insulin-like growth factors-I (IGF-I) and IGF-II. Each have been associated with poor prognosis and more aggressive cancers that exhibit increased metabolism and increased glucose uptake. The insulin receptor (IR) has two splice isoforms IR-A and IR-B: IR-A has a higher affinity for IGF-II comparable to that for insulin, whereas the IR-B isoform predominantly just binds to insulin. In this study, we assessed alterations in the IR-A and IR-B isoform ratio and associated changes in cell proliferation and migration of PCa cell lines following exposure to altered concentrations of glucose and treatment with IGF-II and insulin. We observed that where IR-B predominated insulin had a greater effect on migration than IGF-II and IGF-II was more effective when IR-A was the main isoform. With regard to proliferation IGF-II was more effective than insulin regardless of which isoform was dominant. We assessed the abundance of the IR isoforms both in vivo and in vitro and observed that the majority of the tissue samples and cell lines expressed more IR-A than IR-B. Alterations in the isoforms in response to changes in their hormonal milieu could have a profound impact on how malignant cells behave and play a role in promoting carcinogenesis. A greater understanding of the mechanisms underlying changes in alternative splicing of the IR may provide additional targets for future cancer therapies.
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 8, 2016 |
Publication Date | Sep 28, 2016 |
Deposit Date | Sep 15, 2016 |
Publicly Available Date | Sep 15, 2016 |
Journal | Frontiers in Endocrinology |
Electronic ISSN | 1664-2392 |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | SEP |
DOI | https://doi.org/10.3389/fendo.2016.00132 |
Keywords | insulin, prostate cancer |
Public URL | https://uwe-repository.worktribe.com/output/907881 |
Publisher URL | http://dx.doi.org/10.3389/fendo.2016.00132 |
Contract Date | Sep 15, 2016 |
Files
fendo-07-00132.pdf
(3.5 Mb)
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