Morpholino Oligonucleotide Cross-Linked Hydrogels as Portable Optical Oligonucleotide Biosensors

Langford, Geraint J.; Raeburn, Jaclyn; Ferrier, David C.; Hands, Philip J. W.; Shaver, Michael P.

Abstract

© 2018 American Chemical Society. Morpholino Oligonucleotides (MOs), an uncharged DNA analogue, are functionalized with an acrylamide moiety and incorporated into polymer hydrogels as responsive cross-links for microRNA sequence detection. The MO cross-links can be selectively cleaved by a short target analyte single-stranded DNA (ssDNA) sequence based on microRNA, inducing a distinct swelling response measured optically. The MO cross-links offer significant improvement over DNA based systems through improved thermal stability, no salt requirement and 1000-fold improved sensitivity over a comparative biosensor, facilitating a wider range of sensing conditions. Analysis was also achieved using a mobile phone camera, demonstrating portability.

• Journal Article Type: Article
• Acceptance Date: Dec 28, 2018
• Online Publication Date: Dec 28, 2018
• Publication Date: Jan 25, 2019
• Deposit Date: Jan 25, 2019
• Journal: ACS Sensors
• Print ISSN: 2379-3694
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 1
• Pages: 185-191
• DOI: https://doi.org/10.1021/acssensors.8b01208
• Keywords: morpholino oligonucleotide, oligonucleotide sensor, biosensor, hydrogel, microRNA
• Public URL: https://uwe-repository.worktribe.com/output/853100
• Publisher URL: http://doi.org/10.1021/acssensors.8b01208
• Additional Information: Additional Information : This is the author's accepted manuscript. The final published version is available here: http://doi.org/10.1021/acssensors.8b01208.
• Contract Date: Jan 25, 2019