Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Sage, Ian C.; Drago, Guido A.; Pemberton, Roy; Cox, Timothy; Tuffin, Rachel; Griffiths, John; Pittson, Robin; Johnson, Graham; Xu, Jinsheng; Davies, Rhodri; Jackson, Simon K.; Kenna, Gerry; Luxton, Richard W.; Hart, John P.

doi:10.3390/s141120519

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Sage, Ian C.; Drago, Guido A.; Pemberton, Roy; Cox, Timothy; Tuffin, Rachel; Griffiths, John; Pittson, Robin; Johnson, Graham; Xu, Jinsheng; Davies, Rhodri; Jackson, Simon K.; Kenna, Gerry; Luxton, Richard W.; Hart, John P.

Authors

Ian C. Sage

Guido A. Drago

Roy Pemberton Roy.Pemberton@uwe.ac.uk
Senior Lecturer

Dr Timothy Cox Timothy.Cox@uwe.ac.uk
Research Director

Rachel Tuffin

John Griffiths

Robin Pittson

Graham Johnson

Jinsheng Xu

Rhodri Davies

Simon K. Jackson

Gerry Kenna

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor

John Hart John.Hart@uwe.ac.uk
Professor

Abstract

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

Journal Article Type	Article
Acceptance Date	Oct 21, 2014
Online Publication Date	Oct 30, 2014
Publication Date	Nov 1, 2014
Deposit Date	Dec 15, 2014
Publicly Available Date	Feb 9, 2016
Journal	Sensors (Switzerland)
Electronic ISSN	1424-8220
Publisher	MDPI
Peer Reviewed	Peer Reviewed
Volume	14
Issue	11
Pages	20519-20532
DOI	https://doi.org/10.3390/s141120519
Keywords	microbiosensor, screen-printing, MEMS, 96-well plate, metabolism, amperometry, cell monitoring, toxicity testing
Public URL	https://uwe-repository.worktribe.com/output/808585
Publisher URL	http://dx.doi.org/10.3390/s141120519
Contract Date	Feb 9, 2016