Evaluation of microbial viability and nucleic acid integrity after exposure to new molecular transport medium (Σ-MM™)

Elcocks, Ellena; Qadir, Sheikh; Adukwu, Emmanuel

Abstract

A range of organisms (bacteria and yeasts) were inoculated into Sigma MMTM. The inoculum was then aliquoted into TSB broth at 1 minute and 2 minutes to assess for turbidity indicating growth over a 10 day period. A separate aliquot was also removed for molecular assessment. No turbidity was noted at 10 days for any of the organisms tested. This study demonstrated that the Sigma MMTM molecular medium is able to rapidly inactivate bacteria and yeast whilst preserving bacterial DNA.

• Report Type: Technical Report
• Publication Date: Jul 1, 2022
• Deposit Date: Dec 25, 2022
• Publicly Available Date: Jan 3, 2023
• Keywords: Molecular transport; microbial viability; Nucleic acid integrity; SARS-CoV-2; SARS-CoV-2 Σ-MM™ deactivating and stabilising media
• Public URL: https://uwe-repository.worktribe.com/output/10274453
• Publisher URL: https://www.mwe.co.uk/wp-content/uploads/2022/07/Sigma-MM-Clinical-Studies.pdf