Outputs (34)

The sorting of blood group‐active proteins during enucleation (2015)
Journal Article

Enucleation represents the critical stage during red blood cell development when the nucleus is extruded from an orthochromatic erythroblast in order to generate a nascent immature reticulocyte. Extrusion of the nucleus results in loss of a proportio... Read More about The sorting of blood group‐active proteins during enucleation.

The cytoskeletal binding domain of band 3 is required for multiprotein complex formation and retention during erythropoiesis (2015)
Journal Article

Band 3 is the most abundant protein in the erythrocyte membrane and forms the core of a major multiprotein complex. The absence of band 3 in human erythrocytes has only been reported once, in the homozygous band 3 Coimbra patient. We used in vitro cu... Read More about The cytoskeletal binding domain of band 3 is required for multiprotein complex formation and retention during erythropoiesis.

Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte (2014)
Journal Article

The blood stage malaria parasite, the merozoite, has a small window of opportunity during which it must successfully target and invade a human erythrocyte. The process of invasion is nonetheless remarkably rapid. To date, mechanistic models of invasi... Read More about Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte.

Characteristic phenotypes associated with congenital dyserythropoietic anemia (type II) manifest at different stages of erythropoiesis (2013)
Journal Article

Congenital dyserythropoietic anemia type II is an autosomally recessive form of hereditary anemia caused by SEC23B gene mutations. Patients exhibit characteristic phenotypes including multinucleate erythroblasts, erythrocytes with hypoglycosylated me... Read More about Characteristic phenotypes associated with congenital dyserythropoietic anemia (type II) manifest at different stages of erythropoiesis.

Protein distribution during human erythroblast enucleation in vitro (2013)
Journal Article

Enucleation is the step in erythroid terminal differentiation when the nucleus is expelled from developing erythroblasts creating reticulocytes and free nuclei surrounded by plasma membrane. We have studied protein sorting during human erythroblast e... Read More about Protein distribution during human erythroblast enucleation in vitro.

Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by Epo-withdrawal (2012)
Journal Article

The availability of Erythropoietin (Epo) is essential for the survival of erythroid progenitors. Here we study the effects of Epo removal on primary human erythroblasts grown from peripheral blood CD34+ cells. The erythroblasts died rapidly from apop... Read More about Differential proteomic analysis of human erythroblasts undergoing apoptosis induced by Epo-withdrawal.

Refined views of multi-protein complexes in the erythrocyte membrane (2012)
Journal Article

The erythrocyte membrane has been extensively studied, both as a model membrane system and to investigate its role in gas exchange and transport. Much is now known about the protein components of the membrane, how they are organised into large multi-... Read More about Refined views of multi-protein complexes in the erythrocyte membrane.

Critical band 3 multiprotein complex interactions establish early during human erythropoiesis (2011)
Journal Article

Band 3, the major anion transport protein of human erythrocytes, forms the core of a multiprotein complex in the erythrocyte membrane. Here we studied the spatiotemporal mechanisms of band 3 multiprotein complex assembly during erythropoiesis. Signif... Read More about Critical band 3 multiprotein complex interactions establish early during human erythropoiesis.

Anion exchanger 1 in red blood cells and kidney: Band 3's in a pod (2011)
Journal Article

The bicarbonate/chloride exchanger 1 (AE1, Band 3) is abundantly expressed in the red blood cell membrane, where it is involved in gas exchange and functions as a major site of cytoskeletal attachment to the erythrocyte membrane. A truncated kidney i... Read More about Anion exchanger 1 in red blood cells and kidney: Band 3's in a pod.

The majority of the in vitro erythroid expansion potential resides in CD34- cells, outweighing the contribution of CD34+ cells and significantly increasing the erythroblast yield from peripheral blood samples (2010)
Journal Article

The study of human erythropoiesis in health and disease requires a robust culture system that consistently and reliably generates large numbers of immature erythroblasts that can be induced to differentiate synchronously. We describe a culture method... Read More about The majority of the in vitro erythroid expansion potential resides in CD34- cells, outweighing the contribution of CD34+ cells and significantly increasing the erythroblast yield from peripheral blood samples.