The majority of the in vitro erythroid expansion potential resides in CD34- cells, outweighing the contribution of CD34+ cells and significantly increasing the erythroblast yield from peripheral blood samples

van den Akker, Emile; Satchwell, Timothy J.; Pellegrin, Stephanie; Daniels, Geoff; Toye, Ashley M.

Abstract

The study of human erythropoiesis in health and disease requires a robust culture system that consistently and reliably generates large numbers of immature erythroblasts that can be induced to differentiate synchronously. We describe a culture method modified from Leberbauer et al. (2005) and obtain a homogenous population of erythroblasts from peripheral blood mononuclear cells (PBMC) without prior purification of CD34 + cells. This pure population of immature erythroblasts can be expanded to obtain 4×10 8 erythroblasts from 1×10 8 PBMC after 13-14 days in culture. Upon synchronized differentiation, high levels of enucleation (80-90%) and low levels of cell death (<10%) are achieved. We compared the yield of erythroblasts obtained from PBMC, CD34 + cells or PBMC depleted of CD34 + cells and show that CD34 - cells represent the most significant early erythroid progenitor population. This culture system may be particularly useful for investigating the pathophysiology of anemic patients where only small blood volumes are available. © 2010 Ferrata Storti Foundation.

• Journal Article Type: Article
• Acceptance Date: Mar 9, 2010
• Online Publication Date: Sep 1, 2010
• Publication Date: Sep 1, 2010
• Deposit Date: Jul 11, 2024
• Publicly Available Date: Jul 12, 2024
• Journal: Haematologica
• Print ISSN: 0390-6078
• Electronic ISSN: 1592-8721
• Publisher: Ferrata Storti Foundation
• Peer Reviewed: Peer Reviewed
• Volume: 95
• Issue: 9
• Pages: 1594-1598
• DOI: https://doi.org/10.3324/haematol.2009.019828
• Public URL: https://uwe-repository.worktribe.com/output/12121621