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Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications (2018)
Journal Article
May, J. E., Donaldson, C., Gynn, L., & Ruth Morse, H. (2018). Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications. Mutagenesis, 33(3), 241-251. https://doi.org/10.1093/mutage/gey014

© The Author(s) 2018. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. Mesenchymal stem/stromal cells (MSCs) within the bone marrow (BM) are vitally important in forming the micro-environment supporting haematop... Read More about Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications.

The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model. Mutagenesis, 30(6), 861. https://doi.org/10.1093/mutage/gev074

Developing a novel three-dimensional (3D) in vitro model of the bone marrow microenvironment using bone mar- row mesenchymal stem cells (BM-MSCs) in commercial matrices involves a series of distinct processes. This starts with selecting an appropriat... Read More about The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model.

Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure (2015)
Journal Article
Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2015). Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure. Mutagenesis, 30(6), 874. https://doi.org/10.1093/mutage/gev074

Although gene expression analysis using real-time quan- titative reverse transcription-polymerase chain reaction (RT-qPCR) is an accurate method to evaluate cell activi- ties and phenotypes, the reliability of the analysis depends on the selection of... Read More about Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure.

Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner (2015)
Journal Article
Andrews, S., Morse, H. R., & May, J. E. (2015). Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner. Mutagenesis, 30(6), 867-868. https://doi.org/10.1093/mutage/gev074

Multiple myeloma (MM) is an incurable haematological malignancy with most patients eventually developing ad- vanced, relapsing disease and drug resistance (1,2). This may result from protection by BM mesenchymal cells (BM— MSC) interacting with MM ce... Read More about Bone marrow and myeloma cellular communication protects from genotoxicity in a drug-specific manner.

Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment (2013)
Journal Article
May, J. E., Morse, H. R., Page, K., Soh, M., Wexler, S., Cox, C., …Donaldson, C. (2014). Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment. Mutagenesis, 29(1), 79. https://doi.org/10.1093/mutage/get060

The bone marrow (BM) microenvironment is largely com- prised of mesenchymal stromal cells (MSCs) and is vital in supporting haematopoiesis. Limited studies have indicated that the BM microenvironment is damaged by chemotherapy; however the extent and... Read More about Chemotherapy-induced cytotoxicity and genotoxicity to the bone marrow microenvironment.

Multiple myeloma: The bone marrow microenvironment and its relation to treatment (2013)
Journal Article
Andrews, S. W., Kabrah, S., May, J. E., Donaldson, C., & Morse, H. R. (2013). Multiple myeloma: The bone marrow microenvironment and its relation to treatment. British Journal of Biomedical Science, 70(3), 110-120. https://doi.org/10.1080/09674845.2013.11669945

Multiple myeloma is the most common haematological malignancy yet currently it remains incurable. For decades the mainstay in therapy has been non-targeted approaches including genotoxic agents and immunosuppressants. With myeloma predominantly affec... Read More about Multiple myeloma: The bone marrow microenvironment and its relation to treatment.

Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells (2012)
Journal Article
May, J. E., Morse, H. R., Xu, J., & Donaldson, C. (2012). Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells. Toxicology and Applied Pharmacology, 263(3), 374-389. https://doi.org/10.1016/j.taap.2012.07.013

There is an increasing need for development of physiologically relevant in-vitro models for testing toxicity, however determining toxic effects of agents which undergo extensive hepatic metabolism can be particularly challenging. If a source of such... Read More about Development of a novel, physiologically relevant cytotoxicity model: Application to the study of chemotherapeutic damage to mesenchymal stromal cells.

Alkylating chemotherapeutic agents cyclophosphamide and melphalan cause functional injury to human bone marrow-derived mesenchymal stem cells (2011)
Journal Article
Hows, J., Sanders, K., Kemp, K., Kemp, K. C., Morse, R., Sanders, K. L., …Donaldson, C. (2011). Alkylating chemotherapeutic agents cyclophosphamide and melphalan cause functional injury to human bone marrow-derived mesenchymal stem cells. Annals of Hematology, 90(7), 777-789. https://doi.org/10.1007/s00277-010-1141-8

The adverse effects of melphalan and cyclophosphamide on hematopoietic stem cells are well-known; however, the effects on the mesenchymal stem cells (MSCs) residing in the bone marrow are less well characterised. Examining the effects of chemotherape... Read More about Alkylating chemotherapeutic agents cyclophosphamide and melphalan cause functional injury to human bone marrow-derived mesenchymal stem cells.

Chemotherapy-induced mesenchymal stem cell damage in patients with hematological malignancy (2010)
Journal Article
Hows, J., Wexler, S., Kemp, K., Kemp, K. C., Morse, R., Wexler, S. A., …Donaldson, C. (2010). Chemotherapy-induced mesenchymal stem cell damage in patients with hematological malignancy. Annals of Hematology, 89(7), 701-713. https://doi.org/10.1007/s00277-009-0896-2

Hematopoietic recovery after high-dose chemotherapy (HDC) in the treatment of hematological diseases may be slow and/or incomplete. This is generally attributed to progressive hematopoietic stem cell failure, although defective hematopoiesis may be i... Read More about Chemotherapy-induced mesenchymal stem cell damage in patients with hematological malignancy.

Mesenchymal stem cell response to chemotherapeutic damage (2009)
Presentation / Conference
May, J. E., Xu, J., Morse, R., Avent, N. D., Cox, C., Wexler, S. A., & Donaldson, C. (2009, March). Mesenchymal stem cell response to chemotherapeutic damage. Paper presented at 35th Annual meeting EBMT and 25th Meeting of the Nurses Group

Toxicity testing: The search for an in vitro alternative to animal testing (2009)
Journal Article
Donaldson, C., Morse, H. R., Xu, J., May, J. E., Xu, J., Morse, R., …Donaldson, C. (2009). Toxicity testing: The search for an in vitro alternative to animal testing. British Journal of Biomedical Science, 66(3), 160-165. https://doi.org/10.1080/09674845.2009.11730265

Prior to introduction to the clinic, pharmaceuticals must undergo rigorous toxicity testing to ensure their safety. Traditionally, this has been achieved using in vivo animal models. However, besides ethical reasons, there is a continual drive to red... Read More about Toxicity testing: The search for an in vitro alternative to animal testing.


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