Skip to main content

Research Repository

Advanced Search

All Outputs (30)

BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer (2020)
Journal Article
Shafei, M. A., Forshaw, T., Davis, J., Flemban, A., Qualtrough, D., Dean, S., …Conway, M. E. (2020). BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer. Oncotarget, 11, 1971-1987. https://doi.org/10.18632/oncotarget.27607

The cytosolic branched chain aminotransferase (BCATc) protein has been found to be highly expressed in breast cancer subtypes, including triple negative breast cancer (TNBC), compared with normal breast tissue. The catabolism of branched-chain amino... Read More about BCATc modulates crosstalk between the PI3K/Akt and the Ras/ERK pathway regulating proliferation in triple negative breast cancer.

BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease (2020)
Journal Article
Harris, M., El Hindy, M., Usmari-Moraes, M., Hudd, F., Shafei, M., Dong, M., …Conway, M. E. (in press). BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease. Free Radical Biology and Medicine, https://doi.org/10.1016/j.freeradbiomed.2020.01.019

Leucine, nutrient signal and substrate for the branched chain aminotransferase (BCAT) activates the mechanistic target of rapamycin (mTORC1) and regulates autophagic flux, mechanisms implicated in the pathogenesis of neurodegenerative conditions such... Read More about BCAT-induced autophagy regulates Aβ load through an interdependence of redox state and PKC phosphorylation-implications in Alzheimer's disease.

Novel blood biomarkers that correlate with cognitive performance and hippocampal volumetry: Potential for early diagnosis of Alzheimer's disease (2019)
Journal Article
Hudd, F., Shiel, A., Harris, M., Bowdler, P., McCann, B., Tsivos, D., …Conway, M. E. (2019). Novel blood biomarkers that correlate with cognitive performance and hippocampal volumetry: Potential for early diagnosis of Alzheimer's disease. Journal of Alzheimer's Disease, 67(3), 931-947. https://doi.org/10.3233/JAD-180879

© 2019 - IOS Press and the authors. All rights reserved. Background: Differential diagnosis of people presenting with mild cognitive impairment (MCI) that will progress to Alzheimer's disease (AD) remains clinically challenging. Current criteria used... Read More about Novel blood biomarkers that correlate with cognitive performance and hippocampal volumetry: Potential for early diagnosis of Alzheimer's disease.

Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease (2018)
Journal Article
Usmari Moraes, M., Gaudet, T., Hull, J., & Conway, M. E. (2018). Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease. Bioscience Horizons, 11, 1-7

The development of efficacious treatments targeting Alzheimer’s disease (AD) aetiology has been proved an extensive, time consuming task. Currently prescribed therapies, primarily acetylcholinesterase inhibitors such as donepezil and the NMDAreceptor... Read More about Immunotherapeutic and pharmacological approaches for the treatment of Alzheimer’s disease.

Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line (2017)
Journal Article
Daly, C. S., Flemban, A., Shafei, M., Conway, M. E., Qualtrough, D., & Dean, S. J. (2018). Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line. Oncology Reports, 39(2), 483-490. https://doi.org/10.3892/or.2017.6125

A common feature among pre-malignant lesions is the induction of hypoxia through increased cell propagation and reduced access to blood flow. Hypoxia in breast cancer has been associated with poor patient prognosis, resistance to chemotherapy and inc... Read More about Hypoxia modulates the stem cell population and induces EMT in the MCF-10A breast epithelial cell line.

Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism (2017)
Journal Article
Hull, J., Usmari Moraes, M., Brookes, E., Love, S., & Conway, M. E. (2018). Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism. Neurochemistry International, 112, 49-58. https://doi.org/10.1016/j.neuint.2017.10.014

© 2017 Glutamate is the major excitatory neurotransmitter of the central nervous system, with the branched-chain amino acids (BCAAs) acting as key nitrogen donors for de novo glutamate synthesis. Despite the importance of these major metabolites, the... Read More about Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism.

The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory (2017)
Journal Article
Dillon, S. E., Tsivos, D., Knight, M., McCann, B., Pennington, C., Shiel, A. I., …Coulthard, E. J. (2017). The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory. Scientific Reports, 7, Article 14069. https://doi.org/10.1038/s41598-017-13853-8

© 2017 The Author(s). Both recognition of familiar objects and pattern separation, a process that orthogonalises overlapping events, are critical for effective memory. Evidence is emerging that human pattern separation requires dentate gyrus. Dentate... Read More about The impact of ageing reveals distinct roles for human dentate gyrus and CA3 in pattern separation and object recognition memory.

Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay (2017)
Journal Article
Jackson, S. K., Sattar, A. A., Abate, W., Liu, J., & Conway, M. E. (2017). Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay. Journal of Medical Microbiology, 66(7), 888-897. https://doi.org/10.1099/jmm.0.000510

© 2017 The Authors. Purpose. The Limulus amebocytelysate (LAL) assay is widely used for the screening of lipopolysaccharide (LPS) in parenteral pharmaceuticals. However, correlation of LPS in Gram-negative bacterial infections by LAL assay has been p... Read More about Evaluation of recombinant factor C assay for the detection of divergent lipopolysaccharide structural species and comparison with Limulus amebocyte lysate-based assays and a human monocyte activity assay.

Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation (2017)
Journal Article
Aboo-Bakkar, Z., Bowtell, J. L., Zainie, A., Conway, M. E., Adlam, A. L. R., & Fulford, J. (2017). Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation. Applied Physiology, Nutrition, and Metabolism, 42(7), 773-779. https://doi.org/10.1139/apnm-2016-0550

© 2017, Canadian Science Publishing. All rights reserved. Blueberries are rich in flavonoids, which possess antioxidant and anti-inflammatory properties. High flavonoid intakes attenuate age-related cognitive decline, but data from human intervention... Read More about Enhanced task-related brain activation and resting perfusion in healthy older adults after chronic blueberry supplementation.

Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia (2016)
Journal Article
Hutson, S. M., Kehoe, P. G., Hull, J., Ashby, E. L., Ashby, E., Kierzkowska, M., …Conway, M. E. (2017). Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia. Neurochemical Research, 42(1), 306-319. https://doi.org/10.1007/s11064-016-1855-7

© 2016, The Author(s). Cytosolic and mitochondrial human branched chain aminotransferase (hBCATc and hBCATm, respectively) play an integral role in brain glutamate metabolism. Regional increased levels of hBCATc in the CA1 and CA4 region of Alzheimer... Read More about Altered Expression of Human Mitochondrial Branched Chain Aminotransferase in Dementia with Lewy Bodies and Vascular Dementia.

Regional Increase in the Expression of the BCAT Proteins in Alzheimer's Disease Brain: Implications in Glutamate Toxicity (2015)
Journal Article
Hull, J., Patel, V., El Hindy, M., Lee, C., Odeleye, E., Hezwani, M., …Conway, M. (2015). Regional Increase in the Expression of the BCAT Proteins in Alzheimer's Disease Brain: Implications in Glutamate Toxicity. Journal of Alzheimer's Disease, 45(3), 891-905. https://doi.org/10.3233/JAD-142970

© 2015 - IOS Press and the authors. All rights reserved. Background: The human branched chain aminotransferases (hBCATm, mitochondrial and hBCATc, cytosolic) are major contributors to brain glutamate production. This excitatory neurotransmitter is th... Read More about Regional Increase in the Expression of the BCAT Proteins in Alzheimer's Disease Brain: Implications in Glutamate Toxicity.

Investigation and verification of a bioluminescent biosensor for the quantitation of ara-CTP generation: A biomarker for cytosine arabinoside sensitivity in acute myeloid leukaemia (2014)
Journal Article
Graham Smith, J., Peter Fitzgerald, S., Martin, A., Ann Smith, M., Conway, M., Anderson, E., …Salisbury, V. (2014). Investigation and verification of a bioluminescent biosensor for the quantitation of ara-CTP generation: A biomarker for cytosine arabinoside sensitivity in acute myeloid leukaemia. Biosensors and Bioelectronics, 52, 345-353. https://doi.org/10.1016/j.bios.2013.09.014

A novel whole cell bacterial biosensor, which emits light in response to the active metabolite of cytosine arabinoside (ara-C, cytarabine), ara-CTP, has been investigated and verified. The biosensor has been formulated as an ex vivo assay, designed f... Read More about Investigation and verification of a bioluminescent biosensor for the quantitation of ara-CTP generation: A biomarker for cytosine arabinoside sensitivity in acute myeloid leukaemia.

A novel bioluminescent bacterial biosensor for measurement of Ara-CTP and cytarabine potentiation by fludarabine in seven leukaemic cell lines (2013)
Journal Article
Conway, M., Anderson, E., Smith, M. A., Martin, A., Ruddock, M., Lamont, J., …Salisbury, V. (2013). A novel bioluminescent bacterial biosensor for measurement of Ara-CTP and cytarabine potentiation by fludarabine in seven leukaemic cell lines. Leukemia Research, 37(6), 690-696. https://doi.org/10.1016/j.leukres.2013.02.012

This study evaluates an in vitro biosensor assay capable of detecting the intracellular levels of the tri-phosphorylated form of cytarabine (Ara-CTP) within one working day. The biosensor predicted the response of seven leukaemic cell lines with vary... Read More about A novel bioluminescent bacterial biosensor for measurement of Ara-CTP and cytarabine potentiation by fludarabine in seven leukaemic cell lines.

Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation (2012)
Journal Article
Hull, J., Hindy, M. E., Kehoe, P. G., Chalmers, K., Love, S., & Conway, M. E. (2012). Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation. Journal of Neurochemistry, 123(6), 997-1009. https://doi.org/10.1111/jnc.12044

The branched chain aminotransferase enzymes (BCAT) serve as nitrogen donors for the production of 30% of de novo glutamate synthesis in rat brain. Despite the importance of this major metabolite and excitatory neurotransmitter, the distribution of BC... Read More about Distribution of the branched chain aminotransferase proteins in the human brain and their role in glutamate regulation.

Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase (2012)
Journal Article
Coles, S. J., Hancock, J. T., & Conway, M. E. (2012). Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase. Acta Biochimica et Biophysica Sinica, 44(2), 172-176. https://doi.org/10.1093/abbs/gmr103

The human branched-chain aminotransferase (hBCAT) isoenzymes are CXXC motif redox sensitive homodimers central to glutamate metabolism in the central nervous system. These proteins respond differently to oxidation by H 2O 2, NO, and S-glutathionylati... Read More about Differential redox potential between the human cytosolic and mitochondrial branched-chain aminotransferase.

S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation (2009)
Journal Article
Coles, S. J., Easton, P., Sharrod, H., Hutson, S. M., Hancock, J. T., Patel, V., & Conway, M. E. (2009). S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation. Biochemistry, 48(3), 645-656. https://doi.org/10.1021/bi801805h

Specific proteins with reactive thiol(ate) groups are susceptible to nitric oxide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation. In the present study the effect of NO modification on the functionality... Read More about S-Nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and S-thiolation.

S-nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and s-thiolation (2009)
Journal Article
Coles, S. J., Easton, P., Sharrod, H., Hutson, S. M., Hancock, J. T., Patel, V. B., & Conway, M. E. (2009). S-nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and s-thiolation. Biochemistry, 48(3), 645-656. https://doi.org/10.1021/bi801805h

Specific proteins with reactive thiol(ate) groups are susceptible to nitric oxide (NO) modification, which can result in S-nitrosation, S-thiolation, or disulfide bond formation. In the present study the effect of NO modification on the functionality... Read More about S-nitrosoglutathione inactivation of the mitochondrial and cytosolic BCAT proteins: S-nitrosation and s-thiolation.

Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase (2008)
Journal Article
Coles, S., Hutson, S. M., Poole, L. B., & Conway, M. E. (2008). Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase. Methods in Molecular Biology, 476, 139-152. https://doi.org/10.1007/978-1-59745-129-1_10

The human branched chain aminotransferase enzymes are key regulators of glutamate metabolism in the brain and are among a growing number of redox-sensitive proteins. Studies that use thiol-specific reagents and electrospray ionization mass spectromet... Read More about Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase.

Regulatory control of human cytosolic branched-chain aminotransferase by oxidation and S-glutathionylation and its interactions with redox sensitive neuronal proteins (2008)
Journal Article
Conway, M. E., Coles, S. J., Islam, M. M., & Hutson, S. M. (2008). Regulatory control of human cytosolic branched-chain aminotransferase by oxidation and S-glutathionylation and its interactions with redox sensitive neuronal proteins. Biochemistry, 47(19), 5465-5479. https://doi.org/10.1021/bi800303h

Redox regulation of proteins through oxidation and S-thiolation are important regulatory processes, acting in both a protective and adaptive role in the cell. In the current study, we investigated the sensitivity of the neuronal human cytosolic branc... Read More about Regulatory control of human cytosolic branched-chain aminotransferase by oxidation and S-glutathionylation and its interactions with redox sensitive neuronal proteins.