Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure

Kabrah, S; May, J E; Donaldson, C; Morse, H R

doi:10.1093/mutage/gev074

An exploration of the direct biological targets of molecular hydrogen (2024)
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Molecular hydrogen (H2), supplied either as a gas or in a solution, has been gaining popularity as a treatment for a variety of conditions and diseases. For example, it has been suggested to be beneficial for neurodegenerative diseases, to ease the i... Read More about An exploration of the direct biological targets of molecular hydrogen.

An interplay of gases: oxygen and hydrogen in biological systems (2024)
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Produced by photosynthesis, oxygen (O2) is a fundamentally important gas in biological systems, playing roles as a terminal electron receptor in respiration and in host defence through the creation of reactive oxygen species (ROS). Hydrogen (H2) play... Read More about An interplay of gases: oxygen and hydrogen in biological systems.

Molecular hydrogen as a beneficial postharvest treatment (2023)
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Molecular hydrogen (H2) has been shown to have significant effects in plants, especially during stress responses. H2 treatment can be used as a gas or as an H2-enriched solution, often termed hydrogen-rich water (HRW). Cellular effects of H2 are ofte... Read More about Molecular hydrogen as a beneficial postharvest treatment.

Gamification in biomedical science education: The successful implementation of Resimion, a scenario-based learning tool (2023)
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Introduction: Scenario-based learning and gamification have many advantages in comparison to traditional didactic teaching methods, including development of many higher-level skills such as analysis and evaluation. It is hoped that these simulations... Read More about Gamification in biomedical science education: The successful implementation of Resimion, a scenario-based learning tool.

Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily (2022)
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Hydrogen gas (molecular hydrogen, H2) has significant effects in a range of organisms, from plants to humans. Many inert gases have been reported to have similar effects, and such responses may be most pronounced when cells are stressed. Xenon (Xe),... Read More about Understanding Hydrogen: Lessons to be learned from physical interactions between the inert gases and the globin superfamily.

Development of a paper-based lateral flow Prothrombin assay (2022)
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Disorders of haemostasis result in both excessive bleeding and clotting and are a major global cause of morbidity and mortality, particularly in the developing world. A small number of simple tests can be used to screen and monitor for such dysfuncti... Read More about Development of a paper-based lateral flow Prothrombin assay.

Molecular hydrogen: Is this a viable new treatment for plants in the UK? (2021)
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Despite being trialed in other regions of the world, the use of molecular hydrogen (H2) for enhanced plant growth and postharvest storage of crops has yet to be widely accepted in the UK. The evidence continues to grow that treatment of plants and pl... Read More about Molecular hydrogen: Is this a viable new treatment for plants in the UK?.

Paper-based lateral flow device for the sustainable measurement of human plasma fibrinogen in low-resource settings (2021)
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Fibrinogen concentration is a major determinant of both clotting and bleeding risk. Clotting and bleeding disorders cause extensive morbidity and mortality, particularly in resource-poor and emergency settings. This is exacerbated by a lack of timely... Read More about Paper-based lateral flow device for the sustainable measurement of human plasma fibrinogen in low-resource settings.

Primary mesenchymal stromal cells in co-culture with leukaemic HL-60 cells are sensitised to cytarabine-induced genotoxicity, while leukaemic cells are protected (2021)
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Tumour microenvironments are hallmarked in many cancer types. In haematological malignancies, bone marrow (BM) mesenchymal stromal cells (MSC) protect malignant cells from drug-induced cytotoxicity. However, less is known about malignant impact on su... Read More about Primary mesenchymal stromal cells in co-culture with leukaemic HL-60 cells are sensitised to cytarabine-induced genotoxicity, while leukaemic cells are protected.

Use of machine learning-enabled scenario-based teaching in Haematology and Biomedical Science, as a replacement and supplement to traditional tools and in-person teaching during COVID-19 (2021)
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One of the biggest challenges during the COVID-19 pandemic has been maintaining student engagement and providing the best learning experience with limited face-to-face (FTF) contact, and social distancing requirements. Whilst multiple tools are avail... Read More about Use of machine learning-enabled scenario-based teaching in Haematology and Biomedical Science, as a replacement and supplement to traditional tools and in-person teaching during COVID-19.

Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells (2021)
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Mesenchymal stromal cells (MSC) protect leukaemic cells from drug-induced toxicity within the bone marrow niche, with increasing evidence of leukaemic impact on supportive stroma. The nucleoside analogue, cytarabine (ara-C), is a front-line agent for... Read More about Shedding smart light on the effectiveness of chemotherapy: using Raman spectroscopy and machine learning to differentiate the effects of Cytarabine toxicity and crosstalk of leukaemic and bone marrow stromal cells.

Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease (2019)
Presentation / Conference Contribution

Resistance to therapy remains one of the greatest challenges in the management of the blood and bone marrow (BM) disorder, acute myeloid leukaemia (AML). This study aimed to investigate how leukaemic and BM stromal cells contribute to altered chemo-s... Read More about Leukaemic and bone marrow stromal cells alter cytarabine genotoxicity and cytotoxicity in favour of disease.

Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML). (2019)
Presentation / Conference Contribution

Mesenchymal stromal cells (MSC) are known to protect leukemic cells from drug-induced toxicity within the bone marrow (BM) niche, however, less is known about leukemic impact on supportive MSC. The nucleoside-analogue, cytarabine (Ara-C), is a front-... Read More about Influence of Bone Marrow Stromal and Leukemic Cells on Cytarabine Chemo-Toxicity in Acute Myeloid Leukemia (AML)..

Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications (2018)
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© The Author(s) 2018. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. Mesenchymal stem/stromal cells (MSCs) within the bone marrow (BM) are vitally important in forming the micro-environment supporting haematop... Read More about Chemotherapy-induced genotoxic damage to bone marrow cells: Long-term implications.

Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia (2017)
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Treatment success in leukaemia is impacted by patient resistance to chemotherapy including cytarabine (ara-C) and the anthracycline daunorubicin (DNR) requiring rapid pre-screening testing. DNR is converted to daunorubicinol (DNRol) by carbonyl reduc... Read More about Rapid in vitro testing and characterization of resistance to chemotherapeutic agents daunorubicin and cytarabine in acute myeloid leukaemia.

Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS) (2017)
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Electrochemically activated solutions (ECAS) are of interest as potential disinfectants due to high biocidal activity against many microorganisms. They are widely used clinically in some countries (decontamination of surgical instruments and wounds),... Read More about Cytotoxicity and genotoxicity of electrochemically activated solutions (ECAS).

Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor (2017)
Presentation / Conference Contribution

This study aimed to develop a 2D leukaemia-stroma model of the bone marrow for analysis of stromal-mediated chemo-protection; known to contribute to chemotherapy resistance. The model must facilitate integration of a bioluminescent biosensor HA1 for... Read More about Development of a 2D leukaemia-stroma model for analyses of chemo-toxicity protection using a novel bacterial bioluminescent biosensor.

A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells (2015)
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Decitabine (DAC) and azacytidine (AZA) are cytidine ana- logues and hypomethylating agents used in the manage- ment of patients with myelodysplastic syndrome (MDS), where the main goal is to prevent transformation to acute myeloid leukaemia (AML), wh... Read More about A novel biosensor for prediction of global hypomethylation by decitabine and azacytadine in leukaemic cells.

The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model (2015)
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Developing a novel three-dimensional (3D) in vitro model of the bone marrow microenvironment using bone mar- row mesenchymal stem cells (BM-MSCs) in commercial matrices involves a series of distinct processes. This starts with selecting an appropriat... Read More about The stromal cell line HS-5 is an alternative mesenchymal stem cell source for the development of a 3D bone marrow model.

Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure (2015)
Journal Article

Although gene expression analysis using real-time quan- titative reverse transcription-polymerase chain reaction (RT-qPCR) is an accurate method to evaluate cell activi- ties and phenotypes, the reliability of the analysis depends on the selection of... Read More about Determination of the appropriate gene for real-time PCR analysis of the immotalized stromal cell line HS-5 in 2D and 3D culture following melphalan exposure.

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