V. C. Salisbury
Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores
Salisbury, V. C.; Robinson, Gareth M.; Greenman, John; Reynolds, Darren M.; Lee, S. W.H.
Authors
Gareth Robinson Gareth2.Robinson@uwe.ac.uk
Associate Director (Partnerships & Planning)
John Greenman john.greenman@uwe.ac.uk
Darren Reynolds Darren.Reynolds@uwe.ac.uk
Professor in Health and Environment
S. W.H. Lee
Abstract
Aims: Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species, however, literature revealing the kill-kinetics of these solutions is limited. The aim of the study was to identify the kill-rate and extent of population kill for a range of target species (including endospores) using ECAS generated at the anode (anolyte). Methods and Results: Standard suspensions of methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus atrophaeus spores and Clostridium difficile spores were treated with anolyte in a quantitative suspension assay. For vegetative cells, all concentrations of anolyte tested reduced the viable population to below the detection limit within 10 s. At a concentration of 99%, anolyte produced a log10 reduction factor of greater than five in viable B. atrophaeus endospores within 90 s and reduced numbers of C. difficile endospores to below the experimental detection limit within 20 s at concentrations of 5% or greater. Conclusions: Anolyte was highly effective in killing test-bacteria and spores. The bactericidal efficacy was retained against vegetative cells at dilutions as low as 1% and against C. difficile spores as low as 5%. Significance and Impact of Study: The results of this study demonstrate that ECAS are effective at lower concentrations and act more rapidly than previously reported. Potent bactericidal and sporicidal activity coupled with point-of-use generation, low production-costs and environmental compatibility suggest that acidic ECAS has the potential to be a useful addition to the current armoury of disinfectants. © 2010 The Society for Applied Microbiology.
Journal Article Type | Article |
---|---|
Publication Date | Mar 1, 2010 |
Deposit Date | Nov 18, 2010 |
Journal | Letters in Applied Microbiology |
Print ISSN | 0266-8254 |
Electronic ISSN | 1472-765X |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 50 |
Issue | 3 |
Pages | 289-294 |
DOI | https://doi.org/10.1111/j.1472-765X.2009.02790.x |
Keywords | bactericidal, disinfection, electrochemically activated solutions, sporicidal |
Public URL | https://uwe-repository.worktribe.com/output/981002 |
Publisher URL | http://dx.doi.org/10.1111/j.1472-765X.2009.02790.x |
Contract Date | Sep 28, 2016 |
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