Quantification of unfractionated heparin in human plasma and whole blood by means of novel fluorogenic anti-FXa assays

Castro-L�pez, Vanessa; Harris, Leanne F.; O'Donnell, James S.; Killard, Anthony

Abstract

Novel and sensitive plate-based fluorogenic anti-factor Xa (FXa) assays were investigated to quantify unfractionated heparin (UFH) in human plasma and whole blood within the therapeutic ranges of 0-1.6. U/mL and 0-0.8. U/mL, respectively. Two fluorogenic anti-FXa assay methods were defined for low (0-0.6. U/mL) and high (0.6-1.2. U/mL) pharmacologically relevant UFH concentration ranges in pooled human plasma. In both cases significant differences were observed at intervals of 0.2. U/mL (P0.99, respectively. The assay was also optimized for whole blood which was capable of differentiating UFH concentrations at intervals of 0.2. U/mL (P0.99. The results obtained in this study could assist diagnostic laboratories towards improved monitoring of UFH therapy. © 2011 Elsevier B.V.

• Journal Article Type: Article
• Publication Date: Dec 15, 2011
• Journal: Journal of Pharmaceutical and Biomedical Analysis
• Print ISSN: 0731-7085
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 5
• Pages: 992-997
• DOI: https://doi.org/10.1016/j.jpba.2011.07.025
• Keywords: fluorogenic anti-FXa assay, whole blood, plasma, UFH, monitoring
• Public URL: https://uwe-repository.worktribe.com/output/969565
• Publisher URL: http://dx.doi.org/10.1016/j.jpba.2011.07.025