Malcolm R. Smyth
Chronocoulometric determination of urea in human serum using an inkjet printed biosensor
Smyth, Malcolm R.; Suman, Suman; O'Reilly, Emmet; Kelly, Michele; Killard, Anthony; Morrin, Aoife
Authors
Suman Suman
Emmet O'Reilly
Michele Kelly
Prof. Tony Killard Tony.Killard@uwe.ac.uk
Research Professor in Biomedical Sciences
Aoife Morrin
Abstract
A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (
Citation
Smyth, M. R., Suman, S., O'Reilly, E., Kelly, M., Morrin, A., & Killard, A. (2011). Chronocoulometric determination of urea in human serum using an inkjet printed biosensor. Analytica Chimica Acta, 697(1-2), 98-102. https://doi.org/10.1016/j.aca.2011.04.036
Journal Article Type | Article |
---|---|
Publication Date | Jul 4, 2011 |
Deposit Date | May 4, 2011 |
Publicly Available Date | Nov 15, 2016 |
Journal | Analytica Chimica Acta |
Print ISSN | 0003-2670 |
Electronic ISSN | 1873-4324 |
Publisher | Elsevier |
Peer Reviewed | Not Peer Reviewed |
Volume | 697 |
Issue | 1-2 |
Pages | 98-102 |
DOI | https://doi.org/10.1016/j.aca.2011.04.036 |
Keywords | biosensor, inkjet, polyaniline nanoparticles, urease, chronocoulometry |
Public URL | https://uwe-repository.worktribe.com/output/961088 |
Publisher URL | http://dx.doi.org/10.1016/j.aca.2011.04.036 |
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