Comparison of cytotoxic changes in ECV304 cell through exposure to DMSO and sodium butyrate using impedance spectroscopy

Wang, Zhiyan; Kiely, Janice; Vargas, Angelines Martinez; Luxton, Richard W.

Abstract

This paper decides an impedance spectroscopy measurement system for real-time monitoring of cellular toxicity. The system is used to compare the impedance response of ECV304 cells to two toxicants, dimethyl sulphoxide (DMSO) and sodium butyrate. The responses at different concentration of toxicants were plotted in 3-D impedance spectra and their correlation coefficients were calculated at four time points. Additionally, Phase contrast microscopy images were used to observe the effect of the two toxicants on the morphology change of the cells. The characteristic information of the spectra compared well with the morphological changes of cells, illustrating that the method is useful for monitoring cytotoxicity effects and cell viability.

• Journal Article Type: Article
• Publication Date: Jan 1, 2012
• Journal: Procedia Chemistry
• Print ISSN: 1876-6196
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Pages: 89-99
• DOI: https://doi.org/10.1016/j.proche.2012.10.134
• Keywords: impedance spectroscopy, toxicant
• Public URL: https://uwe-repository.worktribe.com/output/956273
• Publisher URL: http://dx.doi.org/10.1016/j.proche.2012.10.134