Non-linear data analysis of impedance spectroscopy as applied to cellular toxicity systems

Wang, Zhiyan; Kiely, Janice; Luxton, Richard W.

Abstract

This paper introduces an impedance measurement system that can be performed non-destructively to detect cellular toxicity with a cell culture system. This study examines
ECV304 cells grown in culture well and exposed to toxicant
dimethyl sulphoxide (DMSO). The spectra output from the
impendence analyzer can visualize the difference between the
cellular responds at low and high concentration of DMSO
challenge. In order to further process and compare the cellular behavior challenged at three concentrations of DMSO, an equivalent circuit model based on the electrode/insulator/solution interface is described. This paper also investigates Hilbert-Huang transformation (HHT), which is an adaptive analysis technique to non-linear and non-stationary signals. HHT spectra provide a measure of energy in the whole frequency range varies with different concentrations of toxicant.

• Presentation Conference Type: Conference Paper (unpublished)
• Conference Name: International Conference on Modelling, Identification & Control (ICMIC), 2012
• Start Date: Jun 24, 2012
• End Date: Jun 26, 2012
• Publication Date: Jan 1, 2012
• Journal: Modelling, Identification & Control (ICMIC), 2012 Proceedings of International Conference on
• Peer Reviewed: Peer Reviewed
• Pages: 1256 -1262
• Keywords: Hilbert-Huang transform , impedance measurement, equivalent circuit
• Public URL: https://uwe-repository.worktribe.com/output/956263
• Additional Information: Title of Conference or Conference Proceedings : Modelling, Identification & Control (ICMIC), 2012 Proceedings of International Conference on